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Organophosphorous Pesticide Detection in Olive Oil by Using a Miniaturized, Easy-to-Use, and Cost-Effective Biosensor Combined with QuEChERS for Sample Clean-Up
- Source :
- Sensors, Sensors (Basel) 17 (2016): 34. doi:10.3390/s17010034, info:cnr-pdr/source/autori:Fabiana Arduini 1,*, Matteo Forchielli 1, Viviana Scognamiglio 2, Kozitsina Alisa Nikolaevna3, Danila Moscone 1/titolo:Organophosphorous Pesticide Detection in Olive Oil by Using a Miniaturized, Easy-to-Use, and Cost-Effective Biosensor Combined with QuEChERS for Sample Clean-Up/doi:10.3390%2Fs17010034/rivista:Sensors (Basel)/anno:2016/pagina_da:34/pagina_a:/intervallo_pagine:34/volume:17, Sensors; Volume 17; Issue 1; Pages: 34, Sensors, Vol 17, Iss 1, p 34 (2016), Sensors (Basel, Switzerland)
- Publication Year :
- 2016
- Publisher :
- MDPI AG, 2016.
-
Abstract
- Herein, we report a portable electrochemical biosensor based on butyrylcholinesterase (BChE) immobilized on carbon black (CB)-modified screen-printed electrodes (SPEs) for the detection of organophosphorous pesticides in olive oil. The BChE/CB-SPE biosensor was developed to detect paraoxon in standard solutions as well as in olive oil samples previously treated with the QuEChERS method to extract pesticides from the whole fatty matrix. The biosensor shows a linear concentration range of between 20 and 100 ppb for paraoxon both in standard solutions (phosphate buffer 0.05 M) and in olive oil extracts, with a detection limit of 6 ppb in olive oil extract, corresponding to 10% of inhibition. The accuracy of this biosensor in olive oil samples was assessed with olive oil spiked with paraoxon, obtaining satisfactory recovery values.
- Subjects :
- QuEChERS
carbon black
Biosensing Techniques
02 engineering and technology
Standard solution
lcsh:Chemical technology
Quechers
01 natural sciences
Biochemistry
Article
Analytical Chemistry
screen-printed electrodes
organophosphorous pesticides
electrochemical biosensor
butyrylcholinesterase
Matrix (chemical analysis)
Organophosphorus Compounds
olive oil analysis
Limit of Detection
Electrochemistry
medicine
Settore CHIM/01 - Chimica Analitica
lcsh:TP1-1185
Pesticides
Electrical and Electronic Engineering
Olive Oil
Instrumentation
Detection limit
Chromatography
Paraoxon
Chemistry
010401 analytical chemistry
technology, industry, and agriculture
Pesticide
biosensors
021001 nanoscience & nanotechnology
Organophosphorous Pesticide
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Clean-up
0210 nano-technology
Biosensor
medicine.drug
Subjects
Details
- ISSN :
- 14248220
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Sensors
- Accession number :
- edsair.doi.dedup.....347a222aaadd72f1c192552d23ae1714