Phytochemicals Investigation, Chromatographic Evaluation and Antioxidant Activity Assay on the Root Extract of Carrot (Daucus carota)

Daucus carota roots were chosen for this study in order to examine both their phytochemicals and antioxidant activity (free radical scavenging activity). Ethanol was used to extract the plant's roots' powder sample. n-hexane, chloroform, ethyl acetate, and methanol were used to macerate the crude ethanol extract, which was then screened for alkaloids, flavonoids, saponins, tannins, and water-soluble phenols. Thin Layer Chromatography (TLC) analysis was performed on the crude extract as well as on the other fractions. For the optimum separation of the phytochemicals present in the fractions, solvent systems were designed. Chloroform:Ethyl Acetate was chosen as the solvent system for TLC of the n-hexane, methanol, and ethanol fractions (1:1). 100% of chloroform was used for the chloroform fraction. The phytochemical analysis established the presence of water-soluble phenols, saponins, and flavonoids. The results of the TLC study have shown different spots. According to the results of the antioxidant activity test, the ethanol fraction has a higher level of antioxidant activity than the other fractions. Keywords: Daucus carota, Phytochemicals, Antioxidant activity, Thin layer chromatography (TLC). Title: Phytochemicals Investigation, Chromatographic Evaluation and Antioxidant Activity Assay on the Root Extract of Carrot (Daucus carota) Author: Kamalu Garba Sulaiman, Abubakar Suraja Ibrahim, Aminu Rabi'u Doguwa International Journal of Novel Research in Physics Chemistry & Mathematics ISSN 2394-9651 Vol. 9, Issue 3, September 2022 - December 2022 Page No: 61-65 Novelty Journals Website: www.noveltyjournals.com Published Date: 09-November-2022 DOI: https://doi.org/10.5281/zenodo.7307749 Paper Download link (Source) https://www.noveltyjournals.com/upload/paper/Phytochemicals%20Investigation-09112022-3.pdf
International Journal of Novel Research in Physics Chemistry & Mathematics, ISSN 2394-9651, Novelty Journals, Website: www.noveltyjournals.com