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Characterization of royal jelly proteins in both Africanized and European honeybees (Apis mellifera) by two-dimensional gel electrophoresis
- Source :
- Journal of agricultural and food chemistry. 52(1)
- Publication Year :
- 2004
-
Abstract
- In this study, the proteins contained in royal jelly (RJ) produced by Africanized honeybees and European honeybees (Apis mellifera) haven been analyzed in detail and compared using two-dimensional gel electrophoresis, and the N-terminal amino acid sequence of each spot has been determined. Most spots were assigned to major royal jelly proteins (MRJPs). Remarkable differences were found in the heterogeneity of the MRJPs, in particular MRJP3, in terms of molecular weights and isoelectric points between the two species of RJ. Furthermore, during the determination of the N-terminal amino acid sequence of each spot, for the first time, MRJP4 protein has been identified, the existence of which had been only implied by cloning of its cDNA sequence. The presence of heterogeneous bands of glucose oxidase was also identified. Thus, the results suggest that two-dimensional gel electrophoresis provides a suitable method for the qualitative analysis of the proteins contained in RJ derived from different honeybee species.
- Subjects :
- food.ingredient
Immunoblotting
Biology
food
Complementary DNA
Royal jelly
Botany
Africanized bee
Animals
Electrophoresis, Gel, Two-Dimensional
Amino Acid Sequence
Isoelectric Point
Peptide sequence
Glycoproteins
Gel electrophoresis
Two-dimensional gel electrophoresis
Molecular mass
Fatty Acids
RNA-Binding Proteins
General Chemistry
Bees
Europe
Molecular Weight
Isoelectric point
Biochemistry
Africa
Insect Proteins
General Agricultural and Biological Sciences
Subjects
Details
- ISSN :
- 00218561
- Volume :
- 52
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of agricultural and food chemistry
- Accession number :
- edsair.doi.dedup.....3477b31acad0810c598a25095300ba45