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Fluorinated Gold Nanoparticles for Nanostructure Imaging Mass Spectrometry
- Source :
- ACS Nano
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Nanostructure imaging mass spectrometry (NIMS) with fluorinated gold nanoparticles (f-AuNPs) is a nanoparticle assisted laser desorption/ionization approach that requires low laser energy and has demonstrated high sensitivity. Here we describe NIMS with f-AuNPs for the comprehensive analysis of metabolites in biological tissues. F-AuNPs assist in desorption/ionization by laser-induced release of the fluorocarbon chains with minimal background noise. Since the energy barrier required to release the fluorocarbons from the AuNPs is minimal, the energy of the laser is maintained in the low μJ/pulse range, thus limiting metabolite in-source fragmentation. Electron microscopy analysis of tissue samples after f-AuNP NIMS shows a distinct "raising" of the surface as compared to matrix assisted laser desorption ionization ablation, indicative of a gentle desorption mechanism aiding in the generation of intact molecular ions. Moreover, the use of perfluorohexane to distribute the f-AuNPs on the tissue creates a hydrophobic environment minimizing metabolite solubilization and spatial dislocation. The transfer of the energy from the incident laser to the analytes through the release of the fluorocarbon chains similarly enhances the desorption/ionization of metabolites of different chemical nature, resulting in heterogeneous metabolome coverage. We performed the approach in a comparative study of the colon of mice exposed to three different diets. F-AuNP NIMS allows the direct detection of carbohydrates, lipids, bile acids, sulfur metabolites, amino acids, nucleotide precursors as well as other small molecules of varied biological origins. Ultimately, the diversified molecular coverage obtained provides a broad picture of a tissue's metabolic organization.
- Subjects :
- 0301 basic medicine
Nanostructure
Halogenation
Colon
Carbohydrates
General Physics and Astronomy
Photochemistry
01 natural sciences
Mass Spectrometry
Article
Mass spectrometry imaging
Bacteroides fragilis
Bile Acids and Salts
Mice
03 medical and health sciences
chemistry.chemical_compound
Metabolomics
Desorption
Ionization
Animals
General Materials Science
Amino Acids
Perfluorohexane
Nucleotides
Optical Imaging
010401 analytical chemistry
General Engineering
Lipids
Nanostructures
0104 chemical sciences
Mice, Inbred C57BL
Matrix-assisted laser desorption/ionization
030104 developmental biology
chemistry
Colloidal gold
Gold
Sulfur
Subjects
Details
- ISSN :
- 1936086X and 19360851
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- ACS Nano
- Accession number :
- edsair.doi.dedup.....34728aee6970df8e23e17929b11bbdb9