Back to Search
Start Over
Overexpression of microRNA‑486 affects the proliferation and chemosensitivity of mesothelioma cell lines by targeting PIM1
- Source :
- International Journal of Molecular Medicine
- Publication Year :
- 2021
- Publisher :
- Spandidos Publications, 2021.
-
Abstract
- Dysregulated levels of microRNAs (miRNAs or miRs), involved in oncogenic pathways, have been proposed to contribute to the aggressiveness of malignant pleural mesothelioma (MPM). Previous studies have highlighted the downregulation of miRNA miR-486-5p in patients with mesothelioma and the introduction of miRNA mimics to restore their reduced or absent functionality in cancer cells is considered an important therapeutic strategy. The aim of the present study was to evaluate the mechanisms through which miRNAs may influence the functions, proliferation and sensitivity to cisplatin of MPM cells. In the present study, a miR-486-5p mimic was transfected into the H2052 and H28 MPM cell lines, and cell viability, proliferation, apoptosis and mitochondrial membrane potential were monitored. miR-486-5p overexpression led to a clear impairment of cell proliferation, targeting CDK4 and attenuating cell cycle progression. In addition, transfection with miR-486-5p mimic negatively regulated the release of inflammatory factors and the expression of Provirus integration site for Moloney murine leukaemia virus 1 (PIM1). The sensitivity of the cells to cisplatin was enhanced by enhancing the apoptotic effects of the drug and impairing mitochondrial function. On the whole, the present study demonstrates that miR-486-5p may play an important role in MPM treatment by targeting multiple pathways involved in tumour development and progression. These activities may be mostly related to the downregulation of PIM1, a crucial regulator of cell survival and proliferation. Furthermore, these results provide support for the combined use of miR-486-5p with chemotherapy as a therapeutic strategy for MPM.
- Subjects :
- Mesothelioma
0301 basic medicine
CDK4
Cell
PIM1
Antineoplastic Agents
Apoptosis
Biology
03 medical and health sciences
0302 clinical medicine
Proto-Oncogene Proteins c-pim-1
Downregulation and upregulation
Cell Line, Tumor
microRNA
Genetics
medicine
malignant pleural mesothelioma
Humans
Cell Proliferation
Cell growth
Articles
General Medicine
Transfection
Cell cycle
Up-Regulation
Gene Expression Regulation, Neoplastic
miRNA-486-5p
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
cisplatin sensitivity
030220 oncology & carcinogenesis
Cancer cell
Cancer research
Cisplatin
Subjects
Details
- ISSN :
- 1791244X and 11073756
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Medicine
- Accession number :
- edsair.doi.dedup.....347234d725ffa11f7e45416f3b805301