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Selective recognition and enrichment of carbamazepine in biological samples by magnetic imprinted polymer based on reversible addition-fragmentation chain transfer polymerization
- Source :
- Journal of Chromatography A. 1591:62-70
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- A well-defined molecularly imprinted polymer (Fe3O4@CS@MIP) was synthesized via reversible addition-fragmentation chain transfer polymerization for magnetic solid-phase extraction coupled with high-performance liquid chromatography-diode array detector to detect carbamazepine (CBZ) in biological samples. The composition of Fe3O4@CS@MIP was selected by a two-step screening method. 4-vinyl pyridine, divinylbenzene and dimethylformamide were chosen as the functional monomer, cross-linker and porogen, respectively. The imprinted layer was coated on the surface of the chain transfer agent-modified magnetic chitosan nanoparticles. The prepared Fe3O4@CS@MIP was characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller measurement and vibrating sample magnetometer. The results indicated that Fe3O4@CS@MIP had a large surface area (265.8 m2/g), high saturation magnetization (19.88 emu/g) and uniform structure. Besides, the binding property of the Fe3O4@CS@MIP was studied in detail. The Fe3O4@CS@MIP showed high imprinting factor (IF = 4.83) and desirable adsorption capacity (323.10 μmol/g) to CBZ. Under the optimum conditions, the developed method exhibited excellent linearity (R2>0.999) in the range of 0.01–0.5 mg/L and 1.0–30.0 mg/L, and the limits of detection were 1.0 μg/L and 9.6 μg/L for the urine and serum samples, respectively. Good recoveries (88.22%–101.18%) were obtained with relative standard deviations less than 4.83%. This work provided a practical approach for the selective extraction and detection of CBZ in real samples.
- Subjects :
- Polymers
Biochemistry
Polymerization
Analytical Chemistry
Molecular Imprinting
Magnetics
chemistry.chemical_compound
Adsorption
Limit of Detection
Spectroscopy, Fourier Transform Infrared
Fourier transform infrared spectroscopy
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Detection limit
Chromatography
Organic Chemistry
Molecularly imprinted polymer
Reproducibility of Results
Chain transfer
General Medicine
Polymer
Divinylbenzene
Kinetics
Carbamazepine
chemistry
Nanoparticles
Spectrophotometry, Ultraviolet
Nuclear chemistry
Subjects
Details
- ISSN :
- 00219673
- Volume :
- 1591
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography A
- Accession number :
- edsair.doi.dedup.....3443e744881b576045e089eaaa9b1c41