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Effects of D-methionine in mice with noise-induced hearing loss mice
- Source :
- The Journal of International Medical Research, Journal of International Medical Research, Vol 47 (2019)
- Publication Year :
- 2019
- Publisher :
- SAGE Publications, 2019.
-
Abstract
- Objective To study the effects of D-methionine in a mouse model of noise-induced hearing loss (NIHL). Methods We investigated changes in auditory function and microscopic cochlear structure in a mouse model of NIHL, and carried out 4-hydroxynonenal (4-HNE) immunostaining and terminal deoxynucleotidyl transferase dUTP nick-end labeling, and examined expression levels of connexins 26 and 30 by western blot. Results The auditory brainstem response threshold was significantly increased by noise exposure. Noise exposure also damaged the inner and particularly the outer hair cells in the cochlear basement membrane, while histochemistry demonstrated only scattered loss of hair cells in the basement membrane in mice treated with D-methionine before or after noise exposure. D-methionine inhibited apoptosis in the cochlear basement membrane, stria vascularis, and spiral ligament. 4-HNE expression in the basement membrane, stria vascularis, and spiral collateral ligament was increased by noise exposure, but this increase was attenuated by D-methionine. Connexin 26 and connexin 30 expression levels were reduced by noise exposure, and this effect was similarly attenuated by D-methionine administered either before or after noise exposure. Conclusion D-methionine administered before or after noise exposure could rescue NIHL by protecting cochlear morphology, inhibiting apoptosis, and maintaining connexin 26 and 30 expression.
- Subjects :
- 0301 basic medicine
Medicine (General)
medicine.medical_specialty
Hearing loss
cochlea
connexin 26
Audiology
medicine.disease_cause
Biochemistry
D methionine
03 medical and health sciences
Cochlear structure
D-methionine
Mice
R5-920
0302 clinical medicine
Methionine
medicine
otorhinolaryngologic diseases
Evoked Potentials, Auditory, Brain Stem
Animals
Auditory function
Cochlea
business.industry
Biochemistry (medical)
Cell Biology
General Medicine
medicine.disease
Pre-Clinical Research Reports
connexin 30
noise-induced hearing loss
Oxidative Stress
030104 developmental biology
Hearing Loss, Noise-Induced
Female
sense organs
medicine.symptom
business
030217 neurology & neurosurgery
Oxidative stress
Noise-induced hearing loss
Subjects
Details
- Language :
- English
- ISSN :
- 14732300 and 03000605
- Volume :
- 47
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- The Journal of International Medical Research
- Accession number :
- edsair.doi.dedup.....34400fb0b929ede05c17a524018fad31