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Haemolysis and lipemia interfere with resistin and myeloperoxidase BioVendor ELISA assays
- Source :
- Biochemia Medica, Volume 29, Issue 2
- Publication Year :
- 2018
-
Abstract
- Introduction: The aim of our study was to investigate the influence of haemolysis and lipemia on resistin (RES) and myeloperoxidase (MPO) measurement by BioVendor enzyme-linked immunosorbent assays (ELISA). Materials and methods: Blood was taken from healthy volunteers into lithium heparin tubes. Plasma samples were spiked with Lipofundin® emulsion (B. Braun Melsungen AG, Germany) for lipemia interference testing. Haemolysed samples were obtained by drawing aliquots of heparinized blood through a 26 gauge needle. Index of haemolysis (H), lipemia (L) and triglyceride concentration were measured on Abbott Architect c8000. Haemoglobin concentration was measured on Sysmex XN-1000. Concentrations of RES and MPO in all samples were determined with RES and MPO ELISA kits (BioVendor, Czech Republic). All measurements were performed in triplicate. Biases from the native samples were calculated for both analytes and compared with an arbitrary value (e.g. ± 10%). Results: Triglyceride concentration in the investigated samples ranged from 0.57 to 38.23 mmol/L, which corresponds to L index from - 0.01 to 13.77. Haemoglobin concentration in all samples ranged from 0 to 8 g/L which correspond to H index from 0.05 to 8.77. Both MPO and RES showed significant biases at 1 g/L haemoglobin (58.7% and 66.7%, respectively). Also, both MPO and RES showed significant biases at 4.66 mmol/L triglycerides (33.8% and - 12.2%, respectively). Conclusions: Resistin BioVendor assays are affected by haemolysis and lipemia already at low degree of interferent. Haemolysis was found to interfere at 1 g/L haemoglobin for both assays, while lipemia interferes at 4.66 mmol/L of triglycerides.
- Subjects :
- 030213 general clinical medicine
Clinical Biochemistry
Enzyme-Linked Immunosorbent Assay
Hyperlipidemias
haemolysis
lipemia
interferences
resistin
myeloperoxidase
Hemolysis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Healthy volunteers
Humans
Resistin
Peroxidase
Chromatography
Plasma samples
Triglyceride
biology
Chemistry
Biochemistry (medical)
Original Articles
Haemolysis
Healthy Volunteers
3. Good health
030220 oncology & carcinogenesis
Myeloperoxidase
biology.protein
Artifacts
Subjects
Details
- ISSN :
- 18467482 and 13300962
- Volume :
- 29
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemia medica
- Accession number :
- edsair.doi.dedup.....342eaae0e352a5940df0fa2dd421516a