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Effect of KnockOut serum replacement on germ cell development of immature testis tissue culture
- Source :
- Theriogenology. 85(2)
- Publication Year :
- 2014
-
Abstract
- To compare KnockOut serum replacement (KSR) and fetal bovine serum (FBS) for the development of germ cells. Testicular tissues from Sprague-Dawley rats were cultured for 4 weeks in culture media supplemented with FBS or KSR. Tissue area was measured at the beginning and end of the culturing period. Testicular histology, development of the germ cells, and the diameter of seminiferous tubules were analyzed by hematoxylin and eosin staining. After 4 weeks in culture, apoptosis and expression of the stage-specific spermatogenesis marker genes Kit, Sycp3, and Crisp1 were assayed. Tissues cultured in KSR-supplemented media were able to sustain growth and gradually increase seminiferous tubule diameter during the culture period. In addition, spermatogonia, primary spermatocytes, secondary spermatocytes, and round spermatids were observed after 4 weeks in culture, and reverse transcription-PCR confirmed expression of the marker genes. In comparison, tissues cultured in FBS-supplemented media showed dwindling testicular organization, necrotic seminiferous tubules, and expression of Kit, but inconsistent expression of Sycp3 and Crisp1 KnockOut serum replacement outperforms FBS as a growth media supplements for culturing immature spermatogonial tissue culture.
- Subjects :
- 0301 basic medicine
Male
Serum
medicine.medical_specialty
H&E stain
Gene Expression
Apoptosis
Biology
Andrology
Rats, Sprague-Dawley
Tissue Culture Techniques
03 medical and health sciences
Tissue culture
0302 clinical medicine
Food Animals
Spermatocytes
Internal medicine
Gene expression
Testis
medicine
Animals
Small Animals
Spermatogenesis
030219 obstetrics & reproductive medicine
Equine
Seminiferous Tubules
Fetal Blood
Spermatids
Spermatozoa
Spermatogonia
Culture Media
Rats
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Seminiferous tubule
Animal Science and Zoology
Cattle
Germ cell
Fetal bovine serum
Subjects
Details
- ISSN :
- 18793231
- Volume :
- 85
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Theriogenology
- Accession number :
- edsair.doi.dedup.....342223c62a9a9ca56c89a31c250e0741