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Involvement of soil bacteria in ABO blood mistyping
- Source :
- Legal Medicine. 17:128-133
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- The current study investigated whether ABO blood mistyping of human biological samples is induced by soil bacteria. A total of 380 bacterial strains were isolated from 50 discrete soil samples using human blood agar, and glycosidase activity evaluated for all strains using 4-nitropheny glycosides (4-nitrophenyl n-acetyl-α-D-galactosaminide, 4-nitrophenyl-α-D-galactopyranoside, 4-nitrophenyl-α-L-fucopyranoside) as substrates. Thirteen strains possessed α-galactosidase activity, and 16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus. An indirect competitive enzyme-linked immunosorbent assay confirmed seven strains exhibited type B antigen degradation activity. These results demonstrated that 1.8% of the bacteria isolated from soil, were Bacillus sp., possessed galactosidase activity, and had the potential to cause ABO blood mistyping.
- Subjects :
- food.ingredient
Glycoside Hydrolases
Bacillus
Enzyme-Linked Immunosorbent Assay
Biology
ABO Blood-Group System
Pathology and Forensic Medicine
Microbiology
food
Antigen
ABO blood group system
Humans
Agar
Soil Microbiology
Soil bacteria
chemistry.chemical_classification
Glycoside
Galactosidase activity
biology.organism_classification
Molecular biology
Issues, ethics and legal aspects
Blood Grouping and Crossmatching
chemistry
Blood Group Antigens
Bacteria
Subjects
Details
- ISSN :
- 13446223
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- Legal Medicine
- Accession number :
- edsair.doi.dedup.....341dfa793b73e6fa101531da3c04bea8