Back to Search
Start Over
Precipitation methods for high-density lipoprotein cholesterol measurement compared, and final evaluation under routine operating conditions of a method with a low sample-to-reagent ratio
- Source :
- Clinical Chemistry, 43, 663-668, Clinical Chemistry, 43, 4, pp. 663-668
- Publication Year :
- 1997
-
Abstract
- We evaluated six precipitation methods for high-density lipoprotein cholesterol (HDL-chol) determination: the heparin/Mn2+precipitation reagent method (Hep), two variants of the phosphotungstic acid/Mg2+ method (Tung-L and Tung-B), the dextran sulfate 50 000/Mg2+ method (Dex), the PEG 6000 method (PEG), and the PEG 6000/dextran sulfate 15 000 (PEG/Dex) method. The Tung-B and PEG/Dex precipitation methods have a low sample/precipitation reagent volume ratio (16.4 mmol/L (undiluted sample) or >28.0 mmol/L (sample diluted twofold); however, ultrafiltration without dilution was the most accurate method. Results of Tung-B under routine conditions (33 technicians) agreed well with those of the PEG method for 406 normo- and hyperlipidemic plasma samples. By comparison with the HDL-chol method from the Centers for Disease Control and Prevention, the Tung-B method showed a total error of 10.6%, which fulfills the criteria of the National Cholesterol Education Program for HDL-chol analysis. In conclusion, with motivated personnel, Tung-B is a reliable, cost-effective method for routine HDL-chol analysis.
- Subjects :
- Modes of action of therapeutic interventions in familial hypercholesterolemia (FH) in man and animal model
Chromatography
Werkingsmechanismen van therapeutische interventies bij erfelijke vormen van hypercholesterolemie de mens en diermodel
Precipitation (chemistry)
Chemistry
Cholesterol
Biochemistry (medical)
Clinical Biochemistry
technology, industry, and agriculture
Ultrafiltration
Ontwikkeling en kwaliteitsborging in de laboratoriumgeneeskunde
Innovation and Quality assurance in laboratory medicine
Dilution
chemistry.chemical_compound
Biochemistry
Reagent
PEG ratio
polycyclic compounds
lipids (amino acids, peptides, and proteins)
Sample preparation
Quantitative analysis (chemistry)
GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Subjects
Details
- ISSN :
- 00099147
- Database :
- OpenAIRE
- Journal :
- Clinical Chemistry, 43, 663-668, Clinical Chemistry, 43, 4, pp. 663-668
- Accession number :
- edsair.doi.dedup.....33b7c3d41b9563f81419cd40e53d359c