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Efficacy of Ebola Glycoprotein-Specific Equine Polyclonal Antibody Product Against Lethal Ebola Virus Infection in Guinea Pigs
- Source :
- The Journal of Infectious Diseases. 218:S603-S611
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Background Filoviruses including Ebola, Sudan, and other species are emerging zoonotic pathogens representing a significant public health concern with high outbreak potential, and they remain a potential bioterrorism-related threat. We have developed a despeciated equine Ebola polyclonal antibody (E-EIG) postexposure treatment against Ebola virus (EBOV) and evaluated its efficacy in the guinea pig model of EBOV infection. Methods Guinea pigs were infected with guinea pig-adapted EBOV (Mayinga strain) and treated with various dose levels of E-EIG (20-100 mg/kg) twice daily for 6 days starting at 24 h postinfection. The E-EIG was also assessed for neutralization activity against related filoviruses including EBOV strains Mayinga, Kikwit, and Makona and the Bundibugyo and Tai Forest ebolavirus species. Results Treatment with E-EIG conferred 83% to 100% protection in guinea pigs. The results demonstrated a comparable neutralization activity (range, 1:512-1:896) of E-EIG against all tested strains, suggesting the potential for cross-protection with the polyclonal antibody therapeutic. Conclusions This study showed that equine-derived polyclonal antibodies are efficacious against lethal EBOV disease in a relevant animal model. Furthermore, the studies support the utility of the equine antibody platform for the rapid production of a therapeutic product in the event of an outbreak by a filovirus or other zoonotic pathogen.
- Subjects :
- Male
0301 basic medicine
Cross Protection
Guinea Pigs
Supplement Articles
Caviidae
Cross Reactions
Antibodies, Viral
medicine.disease_cause
Neutralization
Guinea pig
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Immunology and Allergy
Horses
030212 general & internal medicine
Glycoproteins
Ebola virus
biology
Outbreak
Hemorrhagic Fever, Ebola
Ebolavirus
biology.organism_classification
Virology
3. Good health
030104 developmental biology
Infectious Diseases
Polyclonal antibodies
biology.protein
Female
Antibody
Taï Forest ebolavirus
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 218
- Database :
- OpenAIRE
- Journal :
- The Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....3382a32e7c7024a608956857ec8c0f5e