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NIR Light-Propelled Janus-Based Nanoplatform for Cytosolic-Fueled microRNA Imaging
- Source :
- ACS Applied Materials & Interfaces. 13:3713-3721
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Various nanoplatforms have been developed to visualize intracellular microRNAs (miRNAs) because of their clinical significance in tumor progression and diagnosis. However, the diffusion-limited motion of the nanoplatforms penalizes the miRNA imaging efficiency in cells. Herein, we fabricated a near-infrared (NIR) light-propelled Janus-based nanoplatform to advance the imaging response. The Janus nanomotor covered with an Au half-shell was loaded by the endocytosis adjuvant of the MnO2 nanosheet for delivering a miRNA-responsive hQN (hairpin DNA quadrangular nanostructure) probe with a catalyzed hairpin assembly (CHA). Once the nanoplatform entered into cells, the MnO2 nanosheet was degraded to Mn2+ by endogenous fuels (such as glutathione) to release the hQN probe. The NIR light irradiation of the nanoplatform generated a heat gradient and thus propelled motion of the nanoplatform. This process accelerated the intracellular reaction of the hQN probe with miRNAs to trigger the cascade CHA amplification with an enhanced fluorescence readout.
- Subjects :
- Nir light
Materials science
Light
02 engineering and technology
010402 general chemistry
Endocytosis
01 natural sciences
Cytosol
microRNA
Humans
General Materials Science
Janus
Nanomotor
Nanosheet
Manganese
Optical Imaging
technology, industry, and agriculture
Hep G2 Cells
021001 nanoscience & nanotechnology
Fluorescence
Nanostructures
0104 chemical sciences
MicroRNAs
Microscopy, Fluorescence
MCF-7 Cells
Biophysics
0210 nano-technology
HeLa Cells
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....33811a2990d3f3a1a93036cbb011bfd1