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Subcultured Mycobacterium tuberculosis isolates on different growth media are fully representative of bacteria within clinical samples
- Source :
- Tuberculosis (Edinburgh, Scotland). 116
- Publication Year :
- 2019
-
Abstract
- Mycobacterium tuberculosis (Mtb) whole genome sequencing (WGS) plays an increasingly important role in tuberculosis diagnosis and research. WGS is typically performed on biobanked isolates obtained by subculture during diagnosis. Genetic variation upon culturing is known to occur in other bacterial species. However, little is understood regarding the impact of different subculture media on genome-wide diversity and variant selection in Mtb. Here we compared WGS derived from direct sequencing of sputa samples to WGS sequences from isolates subcultured on 3 different media. Based on analysis of single nucleotide polymorphisms (SNPs), there was no evidence of variant selection caused by the different culture media used, indicating that subcultured clinical strains can be reliably used to explore genetic determinants of Mtb pathogenesis and epidemiological features.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
DNA, Bacterial
Tuberculosis
030106 microbiology
Immunology
Single-nucleotide polymorphism
Biology
Microbiology
Polymorphism, Single Nucleotide
Mycobacterium tuberculosis
03 medical and health sciences
Tuberculosis diagnosis
Predictive Value of Tests
Genetic variation
medicine
Humans
Tuberculosis, Pulmonary
Whole genome sequencing
Genetics
Bacteriological Techniques
Whole Genome Sequencing
Sputum
Reproducibility of Results
biology.organism_classification
medicine.disease
3. Good health
030104 developmental biology
Infectious Diseases
Subculture (biology)
Bacteria
Subjects
Details
- ISSN :
- 1873281X
- Volume :
- 116
- Database :
- OpenAIRE
- Journal :
- Tuberculosis (Edinburgh, Scotland)
- Accession number :
- edsair.doi.dedup.....336c976810a89b041d8e47054ef349ca