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Applying hydrodynamic pressure to efficiently generate induced pluripotent stem cells via reprogramming of centenarian skin fibroblasts
- Source :
- PLoS ONE, Vol 14, Iss 4, p e0215490 (2019), PLoS ONE
- Publication Year :
- 2019
- Publisher :
- Public Library of Science (PLoS), 2019.
-
Abstract
- Induced pluripotent stem cell (iPSC)-technology is an important platform in medicine and disease modeling. Physiological degeneration and disease onset are common occurrences in the aging population. iPSCs could offer regenerative medical options for age-related degeneration and disease in the elderly. However, reprogramming somatic cells from the elderly is inefficient when successful at all. Perhaps due to their low rates of replication in culture, traditional transduction and reprogramming approaches with centenarian fibroblasts met with little success. A simple and reproducible reprogramming process is reported here which enhances interactions of the cells with the viral vectors that leads to improved iPSC generation. The improved methods efficiently generates fully reprogrammed iPSC lines from 105–107 years old subjects in feeder-free conditions using an episomal, Sendai-Virus (SeV) reprogramming vector expressing four reprogramming factors. In conclusion, dermal fibroblasts from human subjects older than 100 years can be efficiently and reproducibly reprogrammed to fully pluripotent cells with minor modifications to the standard reprogramming procedures. Efficient generation of iPSCs from the elderly may provide a source of cells for the regeneration of tissues and organs with autologous cells as well as cellular models for the study of aging, longevity and age-related diseases.
- Subjects :
- Adult
0301 basic medicine
Somatic cell
Science
Genetic Vectors
Induced Pluripotent Stem Cells
Primary Cell Culture
Biology
Transfection
Sendai virus
Transplantation, Autologous
Viral vector
03 medical and health sciences
Transduction (genetics)
0302 clinical medicine
Induced Pluripotent Stem Cells, Ageing, Pluripotency, Geriatrics
Humans
Cellular Reprogramming Techniques
Vector (molecular biology)
Induced pluripotent stem cell
Cells, Cultured
Skin
Aged, 80 and over
Cloning
Multidisciplinary
Regeneration (biology)
Age Factors
Infant, Newborn
Reproducibility of Results
Fibroblasts
Cellular Reprogramming
Skin Aging
3. Good health
Cell biology
030104 developmental biology
030220 oncology & carcinogenesis
Hydrodynamics
Medicine
Reprogramming
Research Article
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....335b1d058f5366945931a77466ca9b11