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Novel assay for the toxicity evaluation of nanoscale zero-valent iron and derived nanomaterials based on lipid peroxidation in bacterial species
- Source :
- Chemosphere. 213:568-577
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Nano-scale zero-valent iron (nZVI) began attracting research attention in remediation practice in recent decades as a prospective nanomaterial applicable to various contaminated matrices. Despite concerns about the negative effects of nanomaterials on ecosystems, the number of reliable toxicity tests is limited. We have developed a test based on the evaluation of oxidative stress (OS). The test employed the analysis of a typical OS marker (malondialdehyde, MDA), after exposure of six bacterial strains to the tested nanomaterial. We also attempted to use other OS and cell membrane damage assays, including the determination of glutathione and lactate dehydrogenase, respectively. However, we found that the components of these assays interfered with nZVI; therefore, these tests were not applicable. The MDA assay was tested using nZVI and three newly engineered oxide shell nZVI materials with different oxide thicknesses. Six different bacterial species were employed, and the results showed that the test was fully applicable for the concentrations of nanomaterials used in remediation practice (0.1–10 g/L). MDA was produced in a dose-response manner, and the bacteria showed a similar response toward pure pyrophoric nZVI, reaching EC50 values of 0.3–1.1 g/L. We observed different responses in the absolute production of MDA; however, the MDA concentrations were correlated with the cell membrane surfaces of the individual strains (R > 0.75; P 0.95; P
- Subjects :
- Environmental Engineering
Iron
Health, Toxicology and Mutagenesis
Metal Nanoparticles
02 engineering and technology
010501 environmental sciences
01 natural sciences
Lipid peroxidation
chemistry.chemical_compound
Malondialdehyde
Lactate dehydrogenase
Toxicity Tests
Environmental Chemistry
Prospective Studies
0105 earth and related environmental sciences
EC50
chemistry.chemical_classification
Reactive oxygen species
Zerovalent iron
Bacteria
Public Health, Environmental and Occupational Health
Reproducibility of Results
General Medicine
General Chemistry
Glutathione
021001 nanoscience & nanotechnology
Pollution
Nanostructures
Oxidative Stress
chemistry
Toxicity
Lipid Peroxidation
Reactive Oxygen Species
0210 nano-technology
Nuclear chemistry
Subjects
Details
- ISSN :
- 00456535
- Volume :
- 213
- Database :
- OpenAIRE
- Journal :
- Chemosphere
- Accession number :
- edsair.doi.dedup.....334e2d7d942d2b578ebd7f90c1a5d4b6