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Gastric Sleeve Surgery Alleviates Obesity-Associated Insulin Resistance and Suppresses Endoplasmic Reticulum Stress in Adipose Tissue of db/db Mice
- Source :
- Obesity surgery. 29(10)
- Publication Year :
- 2019
-
Abstract
- The aims of this study were to evaluate the effects of gastric sleeve surgery on diabetes remission in db/db mice as well as to determine the underlying mechanisms. Thirty spontaneously obese, diabetic mice (C57BL/Ksj-db/db) were randomly divided into three groups: sleeve gastrectomy group, sham-operated group, and control db/db group. Ten db/m lean mice were used as nondiabetic littermate controls. All mice were sacrificed on day 28. The fasting plasma glucose, serum insulin, lipid profile, and oral glucose tolerance were measured pre- and postoperatively. Inflammatory cytokines (TNF-α and IL-6), endoplasmic reticulum (ER) stress–related markers (GRP78, PERK, IRE-1, and ATF6), and glucose transporter 4 (GLUT4) in the adipose tissue were assayed. Sleeve gastrectomy significantly reduced the body weight and food intake in the db/db mice. This surgery improved glucose and lipid metabolism, as manifested by the decrease in the fasting plasma glucose level and partial restoration of lipid abnormalities. Also, the surgery improved glucose tolerance and alleviated insulin resistance in db/db mice. Sleeve gastrectomy surgery induced downregulation of the inflammatory adipocytokines TNF-α and IL-6; suppressed expression of the ER stress–related markers GRP78, PERK, IRE-1, and ATF-6; and increased the expression and distribution of GLUT4 in adipose tissue of db/db mice. The improvement in glucose tolerance following sleeve gastrectomy is associated with alleviation of insulin resistance, reduction of inflammatory adipocytokine levels, and suppression of ER stress. Further studies are needed to assess whether these effects have a causal role.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Sleeve gastrectomy
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Adipokine
Adipose tissue
Mice, Obese
030209 endocrinology & metabolism
Diabetes Mellitus, Experimental
03 medical and health sciences
Eating
Mice
0302 clinical medicine
Insulin resistance
Downregulation and upregulation
Gastrectomy
Diabetes mellitus
medicine
Animals
Insulin
Obesity
Endoplasmic Reticulum Chaperone BiP
Nutrition and Dietetics
biology
business.industry
Body Weight
Glucose transporter
Glucose Tolerance Test
medicine.disease
Endoplasmic Reticulum Stress
Surgery
Mice, Inbred C57BL
Adipose Tissue
Diabetes Mellitus, Type 2
biology.protein
030211 gastroenterology & hepatology
Insulin Resistance
business
GLUT4
Subjects
Details
- ISSN :
- 17080428
- Volume :
- 29
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Obesity surgery
- Accession number :
- edsair.doi.dedup.....33367f834e720c2a9070d5b3a6147307