Deletion of IER3IP1 in mice

Targeting strategy 1. For conditional knockout, the loxP fragments are usually inserted into the introns downstream of the ATG-containing-exon and removal of the flanked exon(s) will result in frame-shift for protein translation. 2. This design is to conditionally knockout the exon 2 and exon3 by Cre-loxP system. The intron 1 is big and insertion of 5’ loxP element will not interfere ier3ip1 mRNA splicing. 3’loxP will be inserted into intergenic region downstream of ier3ip1 and upstream of the promoter-assoicated elements of Hdhd2.