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Bacterial classification and antibiotic susceptibility testing on an integrated microfluidic platform
- Source :
- Lab on a chip. 21(21)
- Publication Year :
- 2021
-
Abstract
- With the prevalence of bacterial infections and increasing levels of antibiotic resistance comes the need for rapid and accurate methods for bacterial classification (BC) and antibiotic susceptibility testing (AST). Here we demonstrate the use of the fluid handling technique digital microfluidics (DMF) for automated and simultaneous BC and AST using growth metabolic markers. Custom instrumentation was developed for this application including an integrated heating module and a machine-learning-enabled low-cost colour camera for real-time absorbance and fluorescent sample monitoring on multipurpose devices. Antibiotic dilutions along with sample handling, mixing and incubation at 37 °C were all pre-programmed and processed automatically. By monitoring the metabolism of resazurin, resorufin beta-D-glucuronide and resorufin beta-D-galactopyranoside to resorufin, BC and AST were achieved in under 18 h. AST was validated in two uropathogenic E. coli strains with antibiotics ciprofloxacin and nitrofurantoin. BC was performed independently and simultaneously with ciprofloxacin AST for E. coli, K. pneumoniae, P. mirabilis and S. aureus. Finally, a proof-of-concept multiplexed system for breakpoint testing of two antibiotics, as well as E. coli and coliform classification was investigated with a multidrug-resistant E. coli strain. All bacteria were correctly identified, while AST and breakpoint test results were in essential and category agreement with reference methods. These results show the versatility and accuracy of this all-in-one microfluidic system for analysis of bacterial growth and phenotype.
- Subjects :
- Staphylococcus aureus
Serial dilution
medicine.drug_class
Antibiotics
Microfluidics
Biomedical Engineering
Bioengineering
02 engineering and technology
Microbial Sensitivity Tests
Bacterial growth
Biochemistry
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Antibiotic resistance
medicine
Escherichia coli
030304 developmental biology
0303 health sciences
biology
Resazurin
General Chemistry
021001 nanoscience & nanotechnology
biology.organism_classification
3. Good health
Anti-Bacterial Agents
Ciprofloxacin
chemistry
Nitrofurantoin
0210 nano-technology
Bacteria
medicine.drug
Subjects
Details
- ISSN :
- 14730189
- Volume :
- 21
- Issue :
- 21
- Database :
- OpenAIRE
- Journal :
- Lab on a chip
- Accession number :
- edsair.doi.dedup.....33091439ac2d8ab36ea474703169722b