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Rapid characterization of high/low producer CHO cells using matrix-assisted laser desorption/ionization time-of-flight
- Source :
- Rapid communications in mass spectrometry : RCM. 24(9)
- Publication Year :
- 2010
-
Abstract
- An intact-cell mass spectrometry (ICM) method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was evaluated for the screening of stable recombinant Chinese hamster ovary (CHO) cell lines, an important mammalian cell line in bioprocessing. With rapid and simple cell pretreatments, viabilities of cells could be rapidly distinguished on the different fingerprints of mass spectra. Detectable m/z values on cell surfaces and their relative intensities were processed by two biostatistical methods, principle components analysis (PCA) and partial least squares (PLS), with promising results. Discrimination among cell lines with different expressed recombinant proteins or different productivities could be achieved. The ICM method has the advantage of providing multiple parameters simultaneously and possesses the potential to become a powerful method for routine monitoring of bioprocesses.
- Subjects :
- Cell Survival
Analytical chemistry
CHO Cells
Mass spectrometry
Protein Engineering
Analytical Chemistry
Interferon-gamma
Cricetulus
Desorption
Ionization
Cricetinae
Partial least squares regression
Animals
Cluster Analysis
Humans
Bioprocess
Least-Squares Analysis
Spectroscopy
Principal Component Analysis
Chromatography
Chemistry
Chinese hamster ovary cell
Organic Chemistry
Computational Biology
Recombinant Proteins
Cell culture
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Mass spectrum
Subjects
Details
- ISSN :
- 10970231
- Volume :
- 24
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Accession number :
- edsair.doi.dedup.....32f3000ad94d8e7d23245dac636717ab