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Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury
- Source :
- Cells, Vol 10, Iss 500, p 500 (2021), Cells, Volume 10, Issue 3
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Traumatic brain injury (TBI) affects over 3 million individuals every year in the U.S. There is growing appreciation that TBI can produce systemic modifications, which are in part propagated through blood–brain barrier (BBB) dysfunction and blood–brain cell interactions. As such, platelets and leukocytes contribute to mechanisms of thromboinflammation after TBI. While these mechanisms have been investigated in experimental models of contusion brain injury, less is known regarding acute alterations following mild closed head injury. To investigate the role of platelet dynamics and bioenergetics after TBI, we employed two distinct, well-established models of TBI in mice: the controlled cortical impact (CCI) model of contusion brain injury and the closed head injury (CHI) model of mild diffuse brain injury. Hematology parameters, platelet-neutrophil aggregation, and platelet respirometry were assessed acutely after injury. CCI resulted in an early drop in blood leukocyte counts, while CHI increased blood leukocyte counts early after injury. Platelet-neutrophil aggregation was altered acutely after CCI compared to sham. Furthermore, platelet bioenergetic coupling efficiency was transiently reduced at 6 h and increased at 24 h post-CCI. After CHI, oxidative phosphorylation in intact platelets was reduced at 6 h and increased at 24 h compared to sham. Taken together, these data demonstrate that brain trauma initiates alterations in platelet-leukocyte dynamics and platelet metabolism, which may be time- and injury-dependent, providing evidence that platelets carry a peripheral signature of brain injury. The unique trend of platelet bioenergetics after two distinct types of TBI suggests the potential for utilization in prognosis.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
mild TBI
Bioenergetics
Traumatic brain injury
respirometry
Cell
Oxidative phosphorylation
bioenergetics
Article
Mice
03 medical and health sciences
0302 clinical medicine
Internal medicine
Brain Injuries, Traumatic
Leukocytes
medicine
Animals
Humans
Platelet
platelet-neutrophil aggregate
lcsh:QH301-705.5
Hematology
business.industry
neutrophil
General Medicine
medicine.disease
Seahorse
Peripheral
nervous system diseases
Disease Models, Animal
030104 developmental biology
Endocrinology
medicine.anatomical_structure
lcsh:Biology (General)
Closed head injury
business
controlled cortical impact
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 20734409
- Volume :
- 10
- Issue :
- 500
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....32db8eb39a57383c0bf93c120caeefef