Back to Search
Start Over
Modeling Adsorption, Conformation, and Orientation of the Fis1 Tail Anchor at the Mitochondrial Outer Membrane
- Source :
- Membranes; Volume 12; Issue 8; Pages: 752
- Publication Year :
- 2022
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2022.
-
Abstract
- Proteins can be targeted to organellar membranes using a tail anchor (TA), a stretch of hydrophobic amino acids found at the polypeptide carboxyl-terminus. The Fis1 protein (Fis1p), which promotes mitochondrial and peroxisomal division in the yeast Saccharomyces cerevisiae, is targeted to those organelles by its TA. Substantial evidence suggests that Fis1p insertion into the mitochondrial outer membrane can occur without the need for a translocation machinery. However, recent findings raise the possibility that Fis1p insertion into mitochondria might be promoted by a proteinaceous complex. Here, we have performed atomistic and coarse-grained simulations to analyze the adsorption, conformation and orientation of the Fis1(TA). Our results support stable insertion at the mitochondrial outer membrane in a monotopic, rather than a bitopic (transmembrane), configuration. Once inserted in the monotopic orientation, unassisted transition to the bitopic orientation is expected to be blocked by the highly charged nature of the TA carboxyl-terminus and by the Fis1p cytosolic domain. Our results are consistent with a model in which Fis1p does not require a translocation machinery for insertion at mitochondria.
- Subjects :
- FIS1
peptide conformation
mitochondria
mitochondrial outer membrane
tail anchor
molecular dynamics
lipid membrane
BIOGENESIS
Saccharomyces cerevisiae
INSERTION
DNM1P
Filtration and Separation
Mitochondrion
01 natural sciences
WD REPEAT PROTEIN
03 medical and health sciences
0103 physical sciences
Organelle
Chemical Engineering (miscellaneous)
030304 developmental biology
0303 health sciences
010304 chemical physics
biology
Chemistry
Process Chemistry and Technology
FISSION
biology.organism_classification
Transmembrane protein
Cytosol
Membrane
MOLECULAR-DYNAMICS
FORCE-FIELD
Biophysics
1182 Biochemistry, cell and molecular biology
Bacterial outer membrane
GTPASE
HIGH-RESOLUTION
MDV1P
Subjects
Details
- Language :
- English
- ISSN :
- 20770375
- Database :
- OpenAIRE
- Journal :
- Membranes; Volume 12; Issue 8; Pages: 752
- Accession number :
- edsair.doi.dedup.....32d0685d9c9457501891dc5f5271e61f
- Full Text :
- https://doi.org/10.3390/membranes12080752