Back to Search
Start Over
Selective Activation of Cognate SNAREpins by Sec1/Munc18 Proteins
- Source :
- Cell, Cell, Elsevier, 2007, 128 (1), pp.183-195. ⟨10.1016/j.cell.2006.12.016⟩
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- International audience; Sec1/Munc18 (SM) proteins are required for every step of intracellular membrane fusion, but their molecular mechanism of action has been unclear. In this work, we demonstrate a fundamental role of the SM protein: to act as a stimu-latory subunit of its cognate SNARE fusion machinery. In a reconstituted system, mamma-lian SNARE pairs assemble between bilayers to drive a basal fusion reaction. Munc18-1/nSec1, a synaptic SM protein required for neurotrans-mitter release, strongly accelerates this reaction through direct contact with both t-and v-SNAREs. Munc18-1 accelerates fusion only for the cognate SNAREs for exocytosis, therefore enhancing fusion specificity.
- Subjects :
- Munc18 Proteins
Vesicle-Associated Membrane Protein 2
[SDV]Life Sciences [q-bio]
Protein subunit
Amino Acid Motifs
Molecular Sequence Data
Syntaxin 1
Biology
Membrane Fusion
Models, Biological
Exocytosis
General Biochemistry, Genetics and Molecular Biology
Syntaxin binding
Mice
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Amino Acid Sequence
SNARE complex assembly
Neurons
Biochemistry, Genetics and Molecular Biology(all)
Lipid bilayer fusion
Rats
Cell biology
[SDV] Life Sciences [q-bio]
Protein Subunits
Multiprotein Complexes
Mutation
SNARE Proteins
Synaptic vesicle priming
Protein Binding
Subjects
Details
- ISSN :
- 00928674 and 10974172
- Volume :
- 128
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....32bcbbadf09b55b749b767ad6f6d40df