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NPY regulates catecholamine secretion from human adrenal chromaffin cells
- Source :
- CIÊNCIAVITAE, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2001
- Publisher :
- The Endocrine Society, 2001.
-
Abstract
- The aim of the present work was to find out whether NPY synthesized in human adrenal chromaffin cells controls in an autocrine/paracrine fashion the release of catecholamines by these cells. Accordingly, the constitutive and regulated release of both NPY and catecholamines was measured simultaneously in cultured human chromaffin cells. In addition, by using both RT-PCR and a combination of specific agonists and antagonists, we characterized the expression of NPY receptors on these cells as well as their pharmacology. Our results were as follows. 1) Human chromaffin cells constitutively secrete NPY. 2) Nicotine elicits a rapid increase in the release of both catecholamines and NPY; this release of NPY is more sustained than that of catecholamines. 3) RT-PCR shows expression of Y1, Y2, Y4, and Y5 receptor mRNA by chromaffin cells; these receptors are functional, as various receptor specific agonists elicit an increase in intracellular calcium. 4) Peptide YY, in contrast to NPY, is not able to stimulate the release of catecholamines. This finding was corroborated by the observation that no receptor-specific antagonists were able to reduce constitutive catecholamine release, whereas an NPY-immunoneutralizing antibody markedly attenuated the secretion. Taken together, these data suggest that NPY originating from the adrenal medulla locally enhances the secretion of catecholamines, presumably by acting via the putative y3 receptor.
- Subjects :
- Adult
Male
Nicotine
medicine.medical_specialty
Adolescent
Chromaffin Cells
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Biology
Biochemistry
03 medical and health sciences
Catecholamines
0302 clinical medicine
Endocrinology
Internal medicine
Adrenal Glands
mental disorders
medicine
Humans
Neuropeptide Y
Peptide YY
Child
Autocrine signalling
Receptor
Cells, Cultured
030304 developmental biology
0303 health sciences
Biochemistry (medical)
Middle Aged
Neuropeptide Y receptor
humanities
Receptors, Neuropeptide Y
medicine.anatomical_structure
Chromaffin cell
Catecholamine
Female
Signal transduction
Adrenal medulla
030217 neurology & neurosurgery
Signal Transduction
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CIÊNCIAVITAE, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Accession number :
- edsair.doi.dedup.....32a4dd998d6a6c2efeafc6869f57924f