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Mannosylated thiolated paromomycin-loaded PLGA nanoparticles for the oral therapy of visceral leishmaniasis
- Source :
- Nanomedicine. 14:387-406
- Publication Year :
- 2019
- Publisher :
- Future Medicine Ltd, 2019.
-
Abstract
- Aim: The present study evaluates the efficacy of paromomycin (PM)-loaded mannosylated thiomeric nanoparticles for the targeted delivery to pathological organs for the oral therapy of visceral leishmaniasis. Materials & methods: Mannosylated thiolated chitosan (MTC)-coated PM-loaded PLGA nanoparticles (MTC-PLGA-PM) were synthesized and evaluated for morphology, drug release, permeation enhancing and antileishmanial potential. Results: MTC-PLGA-PM were spherical in shape with a size of 391.24 ± 6.91 nm and an encapsulation efficiency of 67.16 ± 14%. Ex vivo permeation indicated 12.73-fold higher permeation of PM with MTC-PLGA-PM against the free PM. Flow cytometry indicated enhanced macrophage uptake and parasite killing in Leishmania donovani infected macrophage model. In vitro antileishmanial activity indicated 36-fold lower IC50 for MTC-PLGA-PM as compared with PM. The in vivo studies indicated 3.6-fold reduced parasitic burden in the L. donovani infected BALB/c mice model. Conclusion: The results encouraged the concept of MTC-PLGA-PM nanoparticles as promising strategy for visceral leishmaniasis.
- Subjects :
- Paromomycin
Antiprotozoal Agents
Biomedical Engineering
Leishmania donovani
Medicine (miscellaneous)
Receptors, Cell Surface
Bioengineering
macromolecular substances
02 engineering and technology
Development
Pharmacology
Chitosan
Mice
03 medical and health sciences
chemistry.chemical_compound
In vivo
medicine
Animals
Lectins, C-Type
General Materials Science
IC50
Cells, Cultured
030304 developmental biology
Mice, Inbred BALB C
0303 health sciences
biology
Chemistry
technology, industry, and agriculture
Flow Cytometry
021001 nanoscience & nanotechnology
biology.organism_classification
medicine.disease
In vitro
Mannose-Binding Lectins
Visceral leishmaniasis
Leishmaniasis, Visceral
Nanoparticles
0210 nano-technology
Mannose Receptor
Ex vivo
medicine.drug
Subjects
Details
- ISSN :
- 17486963 and 17435889
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Nanomedicine
- Accession number :
- edsair.doi.dedup.....327e4748e0800d6019f3e108d23b2b60