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Increased frequencies of Th17 cells and IL17a-producing regulatory T-cells preceding the immunodiscordant response to antiretroviral treatment
- Source :
- The Journal of infection. 76(1)
- Publication Year :
- 2017
-
Abstract
- Summary Background Despite the fact that antiretroviral therapy (cART) suppresses HIV-viremia, an adequate CD4 T-cell recovery is not always achieved (immunodiscordant response to cART). IL17a-producing CD4 T-cells (Th17) constitutes an important subset involved in the preservation of mucosal surfaces integrity, which depletion has been associated with disease progression in HIV-infection. However, whether Th17 frequency at cART initiation is associated with a poor CD4 T-cell recovery has not been yet explored. Our aim was to explore whether the Th17 cells and other IL17a-producing T-cell subsets at cART initiation were associated with a subsequent immunodiscordant response to cART. Methods We selected pre-cART samples of antiretroviral-naive subjects with and without a low CD4 recovery after cART (LR-subjects and HR-subjects, respectively). Peripheral blood mononuclear cells (PBMCs) were stimulated with PMA/ionomycine, and the production of several cytokines including IL17a was analyzed by flow cytometry. Results A trend to higher Th17 ( p = 0.05) and increased frequencies of IL17a-producing Treg ( p = 0.011) was found in LR-subjects before cART onset. Despite increased frequencies of both Treg and Th17 in LR-subject at cART initiation, no alteration of Treg/Th17 ratio was observed. While polifunctional profile of CD4 T-cells was not different, frequencies of CD4 T-cells producing cytokine-combinations including IL17a were increased in LR-subjects. Conclusion Increased frequencies of Th17, IL17a-producing Treg and CD4 T-cells producing specific IL17a-containing combinations of cytokines, precede the immunodiscordant response to cART, suggesting a potential contribution of these subsets in such anomalous response to cART.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
Cart
Adult
Male
medicine.medical_specialty
CD4 antigen
Anti-HIV Agents
HIV Infections
Biology
Peripheral blood mononuclear cell
T-Lymphocytes, Regulatory
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Pharmacotherapy
immune system diseases
Internal medicine
Antiretroviral Therapy, Highly Active
medicine
Antiretroviral treatment
Humans
medicine.diagnostic_test
Interleukin-17
virus diseases
hemic and immune systems
Middle Aged
CD4 Lymphocyte Count
030104 developmental biology
Infectious Diseases
Endocrinology
nervous system
chemistry
Immunology
Cytokines
Th17 Cells
IL17A
Homeostasis
030215 immunology
Subjects
Details
- ISSN :
- 15322742
- Volume :
- 76
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of infection
- Accession number :
- edsair.doi.dedup.....326915abd1d0e9583a140a9c510ffd0a