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Easy calibration of the Light Plate Apparatus for optogenetic experiments
- Source :
- MethodsX, Vol 6, Iss, Pp 1480-1488 (2019)
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Optogenetic systems use genetically-encoded light-sensitive proteins to control and study cellular processes. As the number and quality of these systems grows, there is an increasing need for user-friendly and flexible hardware to provide programmed illumination to cultures of cells. One platform which satisfies this need for a variety of optogenetic systems and organisms is the Light Plate Apparatus (LPA), which delivers a controlled light dose to each well of a 24-well plate. Experimental reproducibility requires appropriate calibration to produce accurate light doses within individual wells of the LPA and between LPAs. In this study, we present an easy and accurate method for calibrating the LPA. In particular, we: • developed a 3D printed adaptor and MATLAB code to allow rapid measurement of irradiance produced by the LPA and subsequent calibration • provide appropriate code and methodology for generating a standard curve for each LPA • demonstrate the utility and accuracy of this method between users and LPAs Method name: Easy calibration of the Light Plate Apparatus for optogenetic experiments, Keywords: Optogenetics, Light Plate Apparatus, Calibration, Saccharomyces cerevisiae, Microbes, Optical power meter
- Subjects :
- 0303 health sciences
3d printed
business.industry
Computer science
Clinical Biochemistry
Matlab code
010501 environmental sciences
Optogenetics
01 natural sciences
Light dose
03 medical and health sciences
Medical Laboratory Technology
Optical power meter
Biochemistry, Genetics and Molecular Biology
Calibration
lcsh:Q
business
lcsh:Science
Computer hardware
030304 developmental biology
0105 earth and related environmental sciences
Subjects
Details
- Language :
- English
- ISSN :
- 22150161
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- MethodsX
- Accession number :
- edsair.doi.dedup.....326418bcc8534164ae02c005d9bc435c