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Iron regulatory protein is involved in the immune defense of the Chinese mitten crab Eriocheir sinensis
- Source :
- Fishshellfish immunology. 89
- Publication Year :
- 2018
-
Abstract
- Iron homeostasis is vital to organismal health; it is maintained by the iron regulatory protein (IRP)-iron-responsive element (IRE) signaling pathway. In the Chinese mitten crab Eriocheir sinensis, EsFer-1 and EsFer-2 reportedly have a putative IRE, but an IRP has not yet been identified. In this study, we successfully amplified the full-length cDNA of EsIRP using gene cloning and rapid amplification of cDNA ends techniques. The length of this cDNA was 4474 bp, and it included a 2682-bp open reading frame encoding 893 amino acids. Using quantitative real-time PCR, mRNA transcripts of EsIRP were detected in various tissues. The highest and lowest expression level was detected in the muscle and gills, respectively. In response to Staphylococcus aureus and Vibrio parahaemolyticus challenge, the transcription level of EsIRP was downregulated and that of EsFer-1 and EsFer-2 was upregulated in hemocytes. EsIRP knockdown resulted in increased expression of both EsFer-1 and EsFer-2. After EsFer-1 and EsFer-2 knockdown, the bacterial clearance ability of E. sinensis against S. aureus and V. parahaemolyticus was impaired. In conclusion, our results suggest that the IRP-IRE signaling pathway plays an important role in the innate immune system response in E. sinensis.
- Subjects :
- Male
Staphylococcus aureus
Brachyura
Aquatic Science
Biology
Arthropod Proteins
Rapid amplification of cDNA ends
Complementary DNA
Environmental Chemistry
Animals
Amino Acid Sequence
Phylogeny
Chinese mitten crab
Regulation of gene expression
Gene knockdown
Base Sequence
Vibrio parahaemolyticus
Gene Expression Profiling
Iron-Regulatory Proteins
General Medicine
biology.organism_classification
Molecular biology
Immunity, Innate
Eriocheir
Open reading frame
Gene Expression Regulation
Sequence Alignment
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....3260be64ae569dd1e202af2240a3fee6