Back to Search
Start Over
Targeted cell delivery into infarcted rat hearts by retrograde intracoronary infusion: distribution, dynamics, and influence on cardiac function
- Source :
- Circulation. 110(11 Suppl 1)
- Publication Year :
- 2004
-
Abstract
- Background— Intracoronary infusion for cell transplantation has potential advantages in disseminating cells globally into the myocardium with less injury over direct intramuscular injection. Arterial route, however, has a risk of coronary embolism and a limitation in cell delivery into ischemic or infarcted areas. We assessed the efficiency of retrograde intracoronary cell implantation into infarcted hearts using a novel rat model. Methods and Results— After left coronary artery ligation in rat, a catheter was inserted into the left cardiac vein, which drains the left ventricular free wall. Through this, 1×10 6 skeletal muscle precursor cells expressing nuclear β-galactosidase were infused retrogradely into the vein. In situ staining demonstrated that β-galactosidase–expressing donor cells had disseminated throughout the left ventricular free wall, including both infarcted and surrounding border areas, at 10 minutes after infusion. At 28 days, in contrast, positively stained multinuclear myotubes were found in border zones, whereas no positive cells were seen in infarcted areas. Measurement of β-galactosidase enzyme activity estimated that 29.8±6.9% of total infused cells were retained within the myocardium at 10 minutes and that this number decreased to 23.7±8.1% at 3 days but rapidly increased thereafter, reaching a plateau at 90.2±17.1% by 14 days. Echocardiography and Langendorff perfusion demonstrated that cell implantation improved cardiac function and dimensions by 28 days, compared with both sham-treated and phosphate-buffered saline-infused infarcted hearts, and this was associated with decreased collagen deposition. Conclusion— Retrograde intracoronary cell transplantation could provide an effective cell delivery into infarcted hearts and could be a useful strategy for treating myocardial infarction.
- Subjects :
- Cardiac function curve
Male
medicine.medical_specialty
Cardiac Catheterization
Vena Cava, Superior
Cell Survival
Heart Ventricles
Myocardial Infarction
Myoblasts
Left coronary artery
Genes, Reporter
Physiology (medical)
Precursor cell
medicine.artery
Internal medicine
medicine
Animals
Infusions, Intra-Arterial
Single-Blind Method
Myocardial infarction
Vein
Ligation
Ultrasonography
Coronary Vein
Cardiac Vein
business.industry
Graft Survival
Stroke Volume
medicine.disease
beta-Galactosidase
Coronary Vessels
Fibrosis
Rats
medicine.anatomical_structure
Lac Operon
Rats, Inbred Lew
cardiovascular system
Cardiology
Collagen
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- ISSN :
- 15244539
- Volume :
- 110
- Issue :
- 11 Suppl 1
- Database :
- OpenAIRE
- Journal :
- Circulation
- Accession number :
- edsair.doi.dedup.....325aaeb4fdc362d0ae65cc341a72f3ba