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Endothelial progenitor cells (EPCs) as gene carrier system for rat model of human glioma
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 1, p e30310 (2012)
- Publication Year :
- 2011
-
Abstract
- Background Due to their unique property to migrate to pathological lesions, stem cells are used as a delivery vehicle for therapeutic genes to tumors, especially for glioma. It is critically important to track the movement, localization, engraftment efficiency and functional capability or expression of transgenes of selected cell populations following transplantation. The purposes of this study were to investigate whether 1) intravenously administered, genetically transformed cord blood derived EPCs can carry human sodium iodide symporter (hNIS) to the sites of tumors in rat orthotopic model of human glioma and express transgene products, and 2) whether accumulation of these administered EPCs can be tracked by different in vivo imaging modalities. Methods and Results Collected EPCs were cultured and transduced to carry hNIS. Cellular viability, differential capacity and Tc-99m uptake were determined. Five to ten million EPCs were intravenously administered and Tc-99-SPECT images were acquired on day 8, to determine the accumulation of EPCs and expression of transgenes (increase activity of Tc-99m) in the tumors. Immunohistochemistry was performed to determine endothelial cell markers and hNIS positive cells in the tumors. Transduced EPCs were also magnetically labeled and accumulation of cells was confirmed by MRI and histochemistry. SPECT analysis showed increased activity of Tc-99m in the tumors that received transduced EPCs, indicative of the expression of transgene (hNIS). Activity of Tc-99m in the tumors was also dependent on the number of administered transduced EPCs. MRI showed the accumulation of magnetically labeled EPCs. Immunohistochemical analysis showed iron and hNIS positive and, human CD31 and vWF positive cells in the tumors. Conclusion EPC was able to carry and express hNIS in glioma following IV administration. SPECT detected migration of EPCs and expression of the hNIS gene. EPCs can be used as gene carrier/delivery system for glioma therapy as well as imaging probes.
- Subjects :
- Pathology
Cellular differentiation
Cancer Treatment
lcsh:Medicine
Antigens, CD34
Diagnostic Radiology
0302 clinical medicine
Molecular Cell Biology
AC133 Antigen
lcsh:Science
Neurological Tumors
Cells, Cultured
0303 health sciences
Multidisciplinary
Symporters
Stem Cells
Glioma
Animal Models
Magnetic Resonance Imaging
3. Good health
Oncology
030220 oncology & carcinogenesis
Cord blood
embryonic structures
cardiovascular system
Medicine
Stem cell
Cellular Types
Radiology
circulatory and respiratory physiology
Research Article
Sodium-iodide symporter
medicine.medical_specialty
Cell Survival
Gene delivery
Biology
03 medical and health sciences
Rats, Nude
Model Organisms
Antigens, CD
Cell Line, Tumor
medicine
Animals
Humans
Progenitor cell
030304 developmental biology
Cell Proliferation
Glycoproteins
lcsh:R
Lentivirus
Endothelial Cells
Cancers and Neoplasms
medicine.disease
Rats
Transplantation
Disease Models, Animal
Cancer research
lcsh:Q
Nuclear Medicine
Peptides
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....324979d4dde2db2cf359ee278ee4fbb4