Antibacterial effects of peptides synthesized based on the sequence of ribosome protein S1

Antibiotic resistance of bacteria is a topical problem on a global scale. Sometimes vigorous human activity leads to an increase in the number of bacteria carrying resistance genes in the environment. Antimicrobial peptides (AMPs) and similar compounds are potential candidates for combating antibiotic-resistant bacteria. Previously, we proposed and successfully tested on Thermus thermophilus a new mechanism of AMP action. This mechanism of directed coaggregation is based on the interaction of a peptide capable of forming fibrils with a target protein. In this work, we discuss the criteria for choosing a target for the targeted action of AMP, describe the features of the "parental" S1 ribosomal proteins T. thermophilus and Escherichia coli and the studied peptides using bioinformatic analysis methods, assess the antimicrobial effect of the synthesized peptides on a model organism of E. coli and cytotoxicity on cells of human fibroblasts. The obtained results will be important for the creation of new AMPs for pathogenic organisms.Antibiotikorezistentnost' bakteriĭ iavliaetsia zlobodnevnoĭ problemoĭ mirovogo masshtaba. Podchas aktivnaia chelovecheskaia deiatel'nost' privodit k uvelicheniiu predstavlennosti v okruzhaiushcheĭ srede bakteriĭ, ustoĭchivykh k deĭstviiu antibiotikov. Antimikrobnye peptidy (AMP) iavliaiutsia potentsial'nymi lekarstvennymi kandidatami dlia bor'by s takimi mikroorganizmami. Ranee nami byl predlozhen i uspeshno aprobirovan na primere Thermus thermophilus novyĭ mekhanizm deĭstviia AMP, osnovannyĭ na napravlennoĭ koagregatsii (vzaimodeĭstvii peptida, sposobnogo formirovat' fibrilly s belkom-mishen'iu). V nastoiashcheĭ rabote rassmotreny kriterii vybora misheni dlia napravlennogo deĭstviia AMP, opisany osobennosti “roditel'skikh” S1 ribosomnykh belkov Thermus thermophilus i Escherichia coli i issleduemykh peptidov s primeneniem metodov bioinformaticheskogo analiza, provedena otsenka antimikrobnogo deĭstviia sintezirovannykh peptidov na model'nom organizme Escherichia coli i tsitotoksichnosti na kletkakh fibroblastov cheloveka.