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Harmine promotes molar root development via SMAD1/5/8 phosphorylation
- Source :
- Biochemical and biophysical research communications. 497(3)
- Publication Year :
- 2017
-
Abstract
- Tooth and bone are major tissues involved in physiological calcification in the body, and they use similar molecular pathways for development, homeostasis, and regeneration. Harmine (HMN) is a natural small compound that stimulates osteoblast differentiation in vitro and in vivo. Here we examined the biological effect of HMN on the postnatal development of molar tooth roots and periodontal tissues. HMN supported the formation of tooth roots and periodontal tissues in developing tooth germs. In tooth germ organ culture, HMN promoted the elongation of Hertwig's epithelial root sheath (HERS) and stimulated cell proliferation in HERS and dental follicle-derived tissues, including dental papillae and dental follicles. HMN stimulated cell proliferation and cell movement of HERS-derived cells without mesenchymal cells in vitro and directly induced the phosphorylation of SMAD1/5/8 protein in HERS-derived cells. Our results indicated that HMN was the first natural small compound to stimulate postnatal development of tooth germs.
- Subjects :
- 0301 basic medicine
Molar
Smad5 Protein
Biophysics
Organ culture
Biochemistry
Smad1 Protein
03 medical and health sciences
0302 clinical medicine
stomatognathic system
Cell Movement
medicine
Animals
Phosphorylation
Tooth Root
Molecular Biology
Cell Proliferation
Cell growth
Chemistry
Regeneration (biology)
Mesenchymal stem cell
Osteoblast
030206 dentistry
Cell Biology
Cell biology
Epithelial root sheath
Mice, Inbred C57BL
stomatognathic diseases
Harmine
030104 developmental biology
medicine.anatomical_structure
Smad8 Protein
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 497
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....32290b817bcb84d5b6aa9053fccda601