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Bacterial communities on electron-beam Pt-deposited electrodes in a mediator-less microbial fuel cell
- Source :
- Environmental sciencetechnology. 42(16)
- Publication Year :
- 2008
-
Abstract
- The content of a bacterial consortium found on an electron beam (e-beam) Pt-deposited electrode in a mediator-less microbial fuel cell (MFC) using glucose and glutamate as fuel is reported in this paper. The e-beam Pt-deposited electrode and electrochemically active bacteria (EAB) consortium were developed to improve the mediator-less MFC performance. Denaturing gradient gel electrophoresis (DGGE), restriction fragment length polymorphism (RFLP), and 16S rRNA sequencing were used to identify the EAB consortia. Sequencing results showed that clone ASP-31 was predominant and was similar to Aeromonas hydrophila, an Fe(III)-reducing and EAB. The phylogenetic tree analysis disclosed the presence of gamma-proteobacteria groups such as Aeromonas genus, Enterobacter asburiae, and Klebsiella oxytoca. These results suggest that MFC performance of the e-beam Pt-deposited electrode with Aeromonas genus consortia dominated by A. hydrophila was higher than other MFCs within a short period. With the e-beam Pt-deposited electrode and Aeromonas genus consortia in the mediator-less MFC, it is possible to increase the efficiency of electron transfer between the bacteria and the electrode.
- Subjects :
- DNA, Bacterial
Paper
Microbial fuel cell
Bioelectric Energy Sources
Electrons
Polymerase Chain Reaction
Microbiology
RNA, Ribosomal, 16S
Electrochemistry
Environmental Chemistry
Electrophoresis, Gel, Two-Dimensional
Electrodes
Phylogeny
Platinum
biology
Bacteria
Klebsiella oxytoca
General Chemistry
16S ribosomal RNA
biology.organism_classification
Carbon
Aeromonas hydrophila
Aeromonas
Microscopy, Electron, Scanning
Restriction fragment length polymorphism
Temperature gradient gel electrophoresis
Subjects
Details
- ISSN :
- 0013936X
- Volume :
- 42
- Issue :
- 16
- Database :
- OpenAIRE
- Journal :
- Environmental sciencetechnology
- Accession number :
- edsair.doi.dedup.....3213244eea11c0995061075df836b53a