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Spatial expression and characterization of a putative ethylene receptor protein NTHK1 in tobacco
- Source :
- Plantcell physiology. 43(7)
- Publication Year :
- 2002
-
Abstract
- A putative ethylene receptor gene NTHK1 encodes a protein with a putative signal peptide, three transmembrane segments, a putative histidine kinase domain and a putative receiver domain. The receiver domain was expressed in an Escherichia coli expression system, purified and used to generate polyclonal antibodies for immunohistochemistry analysis. The spatial expression of the NTHK1 protein was then investigated. We found that NTHK1 was abundant during flower and ovule development. It was also expressed in glandular hairs, stem, and in leaves that had been wounded. The NTHK1 gene was further introduced into the tobacco plant and we found that, in different transgenic lines, the NTHK1 gene was transcribed to various degrees. Upon ACC treatment, the etiolated transgenic seedlings showed reduced ethylene sensitivity when compared with the control, indicating that NTHK1 is a functional ethylene receptor in plants.
- Subjects :
- Signal peptide
Physiology
Transgene
Amino Acids, Cyclic
Receptors, Cell Surface
Plant Science
medicine.disease_cause
Gene Expression Regulation, Plant
Tobacco
medicine
Escherichia coli
Receptor
Gene
Plant Proteins
biology
Histidine kinase
Gene Expression Regulation, Developmental
Cell Biology
General Medicine
Ethylenes
Plants, Genetically Modified
Molecular biology
Immunohistochemistry
Transmembrane protein
Phenotype
Polyclonal antibodies
biology.protein
Subjects
Details
- ISSN :
- 00320781
- Volume :
- 43
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Plantcell physiology
- Accession number :
- edsair.doi.dedup.....320c45efc4431cc0a0ced351809d7513