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A metaphyseal fracture rat model for mechanistic studies of osteoporotic bone healing
- Source :
- European Cells & Materials, Vol 37, Pp 420-430 (2019)
- Publication Year :
- 2019
- Publisher :
- European Cells and Materials, 2019.
-
Abstract
- Most osteoporotic fractures occur at metaphyseal regions of long bones. The present study proposed a clinically relevant animal model that satisfied: i) induction of osteoporosis, ii) unilateral complete osteotomy at metaphysis, iii) internal fixation. 6 months old female Sprague-Dawley rats (n = 64) were randomly divided into the ovariectomised-metaphyseal osteotomy (OVX, n = 32) and metaphyseal osteotomy (SHAM, n = 32) groups. The metaphyseal-osteotomy model was created with a plate-fixation of the osteotomy and assessed by X-ray, micro-computed tomography, histomorphometry and mechanical testing at weeks 1, 3 and 6. X-ray results showed complete healing of metaphyseal osteotomy at week 6. Histology showed 3 stages of metaphyseal healing. Stage 1 was characterised by fibrous tissue, consisting of disorganised orientation of collagen fibres, and infiltration of immune cells. At stage 2, a transitional zone consisting of maturing fibrous tissue and differentiating mesenchymal cells with early trabecular bone formation and disorganised woven bone were observed. During stage 3, cortical bone ends unified and woven bone underwent transformation to lamellar bone. OVX group healing was significantly delayed when compared to SHAM samples. The study demonstrated that healing of osteoporotic osteotomy at the metaphyseal region was delayed in terms of radiography, histomorphometry and mechanical strength. These quantitative evaluations, along with histological features, may provide key references for future studies. The animal model may provide additional clinical relevance as most osteoporotic fracture in humans occurs at metaphyseal regions.
- Subjects :
- lcsh:Diseases of the musculoskeletal system
medicine.medical_treatment
Radiography
0206 medical engineering
Osteoporosis
lcsh:Surgery
02 engineering and technology
Metaphysis
Osteotomy
Bone and Bones
Rats, Sprague-Dawley
Fracture Fixation, Internal
Fracture fixation
Animals
Medicine
Internal fixation
metaphyseal fracture
Osteoporotic fracture healing
Fracture Healing
business.industry
Histology
lcsh:RD1-811
X-Ray Microtomography
Anatomy
medicine.disease
020601 biomedical engineering
Rats
Disease Models, Animal
medicine.anatomical_structure
intramembranous ossification
Female
Cortical bone
lcsh:RC925-935
business
endochondral ossification
Osteoporotic Fractures
Subjects
Details
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- European Cells and Materials
- Accession number :
- edsair.doi.dedup.....31d51d5f09334e8fd40528675217270b