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Construction of a novel mRNA-miRNA-lncRNA network and identification of potential regulatory axis associated with prognosis in colorectal cancer liver metastases
- Source :
- Aging (Albany NY)
- Publication Year :
- 2021
- Publisher :
- Impact Journals, 2021.
-
Abstract
- Liver metastasis is a leading cause of death in patients with colorectal cancer (CRC). Increasing evidence demonstrates that competing endogenous RNA (ceRNA) networks play important roles in malignant cancers. The purpose of this study was to identify molecular markers and build a ceRNA network as a significant predictor of colorectal liver metastases (CRLM). By integrated bioinformatics analysis, we found that apolipoprotein C1 (APOC1) was upregulated in CRLM and associated with prognosis in patients with CRC and thereby established an APOC1-dependent ceRNA network. By survival analysis, expression analysis, and correlation analysis of each element in the ceRNA network, we identified that ZEB1-AS1, miR-335-5p and APOC1 regulated each other. We further experimentally confirmed that ZEB1-AS1 promoted a CRC progression via regulating the expression of miR-335-5p that controlled the expression of APOC1. Our findings indicate that the ZEB1-AS1-miR-335-5p-APOC1 ceRNA regulatory network is significantly valuable for better prognosis of patients with CRC and as a new therapeutic target for the treatment of CRLM.
- Subjects :
- Aging
bioinformatics analysis
Colorectal cancer
colorectal cancer liver metastases
Metastasis
competing endogenous RNA (ceRNA)
Downregulation and upregulation
Cell Movement
Cell Line, Tumor
microRNA
medicine
Humans
Gene Regulatory Networks
Neoplasm Invasiveness
RNA, Messenger
Survival analysis
Cause of death
Apolipoprotein C-I
Base Sequence
business.industry
Competing endogenous RNA
Gene Expression Profiling
Liver Neoplasms
Cell Biology
medicine.disease
Prognosis
Survival Analysis
Gene Expression Regulation, Neoplastic
MicroRNAs
Gene Ontology
Cancer research
RNA, Long Noncoding
Apolipoprotein C1
business
Colorectal Neoplasms
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 19454589
- Volume :
- 13
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Aging (Albany NY)
- Accession number :
- edsair.doi.dedup.....31ceeacc12bc4ee12c48a70c7bbf482f