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Comparative sensitivity of proliferative and differentiated intestinal epithelial cells to the food contaminant, deoxynivalenol
- Source :
- Environmental Pollution, Environmental Pollution, Elsevier, 2021, 277, pp.116818. ⟨10.1016/j.envpol.2021.116818⟩
- Publication Year :
- 2020
-
Abstract
- International audience; The intestinal epithelium is a functional and physical barrier formed by a cell monolayer that constantly differentiates from a stem cell in the crypt. This is the first target for food contaminants, especially mycotoxins. Deoxynivalenol (DON) is one of the most prevalent mycotoxins. This study compared the effects of DON (0-100 μM) on proliferative and differentiated intestinal epithelial cells. Three cell viability assays (LDH release, ATP content and neutral red uptake) indicated that proliferative Caco-2 cells are more sensitive to DON than differentiated ones. The establishment of transepithelial electrical resistance (TEER), as a read out of the differentiation process, was delayed in proliferative cells after exposure to 1 μM DON. Transcriptome analysis of proliferative and differentiated exposure to 0-3 μM DON for 24 h revealed 4862 differentially expressed genes (DEG) and indicated an effect of both the differentiation status and the DON treatment. KEGG enrichment analysis indicated involvement of metabolism, ECM receptors and tight junctions in the differentiation process, while ribosome biogenesis, mRNA surveillance, and the MAPK pathway were involved in the response to DON. The number of differentially expressed genes and the amplitude of the effect were higher in proliferative cells exposed to DON than that in differentiated cells. In conclusion, our study shows that proliferative cells are more susceptible than differentiated ones to DON and that the mycotoxin delays the differentiation process.
- Subjects :
- 010504 meteorology & atmospheric sciences
Health, Toxicology and Mutagenesis
Cellular differentiation
Cytotoxicity
[SDV]Life Sciences [q-bio]
Cell
010501 environmental sciences
Biology
Toxicology
Intestinal barrier function
01 natural sciences
Transcriptome
medicine
Humans
Viability assay
Cell proliferation
0105 earth and related environmental sciences
Tight junction
Cell growth
Cell Differentiation
Epithelial Cells
General Medicine
Pollution
Intestinal epithelium
Intestinal cell renewal
Cell biology
Cell toxicity
medicine.anatomical_structure
Mycotoxin
[SDV.TOX]Life Sciences [q-bio]/Toxicology
Stem cell
Caco-2 Cells
Trichothecenes
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Subjects
Details
- ISSN :
- 18736424 and 02697491
- Volume :
- 277
- Database :
- OpenAIRE
- Journal :
- Environmental pollution (Barking, Essex : 1987)
- Accession number :
- edsair.doi.dedup.....31b680172cd30da418ba0e5688a50773