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Neutrophil Activation and Early Features of NET Formation Are Associated With Dengue Virus Infection in Human
- Source :
- Frontiers in Immunology, Frontiers in Immunology, 2019, 9, pp.3007. ⟨10.3389/fimmu.2018.03007⟩, Frontiers in Immunology, Frontiers, 2019, 9, pp.3007. ⟨10.3389/fimmu.2018.03007⟩, Frontiers in Immunology, Vol 9 (2019)
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; The involvement of the immune system in the protection and pathology of natural dengue virus (DENV) has been extensively studied. However, despite studies that have referred to activation of neutrophils in DENV infections, the exact roles of neutrophils remain elusive. Here, we explored the phenotypic and functional responses of neutrophils in a cohort of adult dengue patients. Results indicated that during an acute DENV infection, neutrophils up-regulate CD66b expression, and produce a more robust respiratory response as compared with that in convalescent or healthy individuals; this confirmed in vivo neutrophil activation during DENV infection. Spontaneous decondensation of nuclei, an early event of neutrophil extracellular trap (NET) formation, was also markedly increased in cells isolated from DENV-infected patients during the acute phase of the infection. In vitro incubation of NETs with DENV-2 virus significantly decreased DENV infectivity. Interestingly, increased levels of NET components were found in the serum of patients with more severe disease form-dengue hemorrhagic fever (DHF), but not uncomplicated dengue fever, during the acute phase of the infection. Levels of pro-inflammatory cytokines IL-8 and TNFα were also increased in DHF patients as compared with those in healthy and DF subjects. This suggested that NETs may play dual roles during DENV infection. The increased ability for NET formation during acute DENV infection appeared to be independent of PAD4-mediated histone H3 hyper-citrullination. Our study suggests that neutrophils are involved in immunological responses to DENV infection.
- Subjects :
- 0301 basic medicine
Neutrophils
viruses
Dengue virus
medicine.disease_cause
Extracellular Traps
Neutrophil Activation
Dengue fever
Cohort Studies
0302 clinical medicine
Aedes
Immunology and Allergy
Medicine
innate immunity
Original Research
Infectivity
virus diseases
neutrophil
Thailand
3. Good health
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Cytokines
[SDV.IMM]Life Sciences [q-bio]/Immunology
Adult
lcsh:Immunologic diseases. Allergy
Immunology
GPI-Linked Proteins
Virus
Cell Line
03 medical and health sciences
Immune system
Antigens, CD
In vivo
Animals
Humans
Severe Dengue
Innate immune system
business.industry
NETs
Neutrophil extracellular traps
Dengue Virus
biochemical phenomena, metabolism, and nutrition
medicine.disease
dengue
NETs Opasawatchai et al NETs in DENV Infection
030104 developmental biology
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
viral infection
lcsh:RC581-607
business
Cell Adhesion Molecules
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology, Frontiers in Immunology, 2019, 9, pp.3007. ⟨10.3389/fimmu.2018.03007⟩, Frontiers in Immunology, Frontiers, 2019, 9, pp.3007. ⟨10.3389/fimmu.2018.03007⟩, Frontiers in Immunology, Vol 9 (2019)
- Accession number :
- edsair.doi.dedup.....31af2e83dc719c24ef358d865fac6b60