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IN VIVO EVALUATION OF TIMING, DEGREE, AND DISTRIBUTION OF BACTERIAL TRANSLOCATION FOLLOWING EXPERIMENTAL SMALL BOWEL TRANSPLANTATION
- Source :
- Transplantation. 60:891-896
- Publication Year :
- 1995
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1995.
-
Abstract
- These studies were designed to evaluate the correlation between morphologic and functional changes after heterotopic auxiliary small bowel isograft with systemic venous drainage and two ostomies in 20 Lewis rats. Morphologic damage of the graft was scored by full-thickness biopsies before surgery and 1, 3, 5, and 7 days after transplant. Functional evaluation of the graft was done, at the same time points, by urinary excretion of lactulose and mannitol injected in the proximal ostomy. The intestinal permeability was also studied by injecting Escherichia coli labeled with indium-111 oxine in the proximal ostomy. Translocation of radiolabeled bacteria was quantitated in extraintestinal tissues by radionuclide counts and number of viable organisms and in vivo by scintigraphic imaging. One day after transplant, significant graft damage (score 17.2 +/- 4.2) was observed when compared with the pretransplant value (7.3 +/- 2.6). The degree of tissue injury was similar on days 3 (15.8 +/- 3.5) and 5 (16.1 +/- 3.9) after transplant and remained high on day 7 (11.8 +/- 2.8). The lactulose to mannitol ratio showed a significantly increased permeability on day 1 (17.5) versus pretransplant values (2.6), remained high on day 3 (8.6), and returned to normal values on day 5 (2.8). Translocation of bacteria to distant organs, as measured by both radionuclide counts and number of viable organisms, was strikingly enhanced on day 1 after transplantation, compared with control animals, but returned to the pretransplant value on day 3. A good qualitative and quantitative correlation was observed between radionuclide counts in the extraintestinal organs and in vivo images obtained by scintigraphic scanning. In conclusion, in this model, timing and degree of bacterial translocation do not seem to correlate well, with the exception of the acute post-transplantation phase, with morphologic and permeability changes of the graft. Evaluation of translocation by scintigraphic imaging appears a suitable approach to study in vivo the kinetics and distribution of this process.
- Subjects :
- Male
medicine.medical_specialty
Pathology
Time Factors
Time Factor
Isograft
Escherichia coli Infection
Chromosomal translocation
Biology
Gastroenterology
Permeability
Lactulose
Postoperative Complications
Indium Radioisotope
In vivo
Internal medicine
Intestine, Small
Escherichia coli
Organometallic Compounds
medicine
Animals
Mannitol
Lung
Escherichia coli Infections
Organometallic Compound
Transplantation
Intestinal permeability
Animal
Graft Survival
Indium Radioisotopes
Oxyquinoline
medicine.disease
Small intestine
Rats
Transplantation, Isogeneic
medicine.anatomical_structure
Liver
Rats, Inbred Lew
Rat
Postoperative Complication
medicine.drug
Subjects
Details
- ISSN :
- 00411337
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Transplantation
- Accession number :
- edsair.doi.dedup.....31aa28ac7867980efbb6462633a31859