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Reactive oxygen intermediates cause rapid release of the interleukin-1 decoy receptor from human myelomonocytic cells
- Source :
- Scopus-Elsevier
- Publication Year :
- 1996
-
Abstract
- Free radicals play an important role in inflammation. We found that reactive oxygen intermediates (ROI) inhibit interleukin-1beta (IL- 1beta) binding on human myelomonocytes. Production of superoxide anion (O2-) by Xanthine (X) and Xanthine-Oxidase (XO) or NADPH caused a reduction (48% +/- 15% in 25 experiments) in the IL-1beta binding of polymorphonuclear cells (PMN) and monocytes that was inhibited by superoxide dismutase (SOD). Hydrogen peroxide (H2O2) was only active on monocytes and this effect was prevented by catalase. O2(-)-induced loss of IL-1beta binding on PMN reached half maximum at 5 minutes and peaked after 30 minutes. The reduction of IL-1beta binding was due to reduction of IL-1beta receptors (R) on PMN surface without any change in affinity. ROI-induced reduction of surface IL-1R was not caused by receptor internalization, but rather by the release of a soluble form (45 kD) of the type II decoy R. The action of ROI on IL-1 binding was selective because major histocompatibility complex class I, CD18 and CD16 were unaffected. The O2(-)-induced release of IL-1 decoy R was not affected by protein synthesis inhibitors, but was partially blocked by protease inhibitors. Release of the IL-1 type II decoy R might represent one mechanism by which ROI antagonize and limit the proinflammatory effects of IL-1.
- Subjects :
- Xanthine Oxidase
Neutrophils
Immunology
CD16
Biochemistry
Xanthine
Monocytes
Proinflammatory cytokine
Superoxide dismutase
chemistry.chemical_compound
medicine
Humans
Receptor
Cells, Cultured
biology
Superoxide
Superoxide Dismutase
Monocyte
Receptors, Interleukin-1
Cell Biology
Hematology
Hydrogen Peroxide
Endocytosis
Recombinant Proteins
medicine.anatomical_structure
chemistry
Catalase
Xanthines
biology.protein
Decoy
Reactive Oxygen Species
Oxidation-Reduction
NADP
Interleukin-1
Subjects
Details
- ISSN :
- 00064971
- Volume :
- 87
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....3195b290ea14fe5fb1d2804beccd2172