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An advanced method for the immunohistochemical detection of nitric oxide synthase (NOS) in the female genital tract
- Source :
- Analytical Biochemistry. 631:114264
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The expression of nitric oxide synthase (NOS) in male and female urogenital tissues has been investigated by using conventional light microscopical immunoperoxidase staining. We present an improved immunohistochemical method for the specific and simultaneous detection of endothelial and neuronal NOS (eNOS/nNOS) in vaginal tissue. Specific antibodies have been used in combination with the tyramide signal amplification method. We found a subepithelial meshwork of varicose nerve fibers. A subpopulation of fibers presented immunoreactivity specific for nNOS. Epithelial cells also showed cytoplasmatic labeling for nNOS. Arteries presenting signals for eNOS in their endothelial layer were found in close proximity to nNOS-positive nerve fibers.
- Subjects :
- Female circumcision
Pathology
medicine.medical_specialty
Nitric Oxide Synthase Type III
Biophysics
Nitric Oxide Synthase Type I
01 natural sciences
Biochemistry
03 medical and health sciences
Enos
medicine
Humans
Molecular Biology
030304 developmental biology
0303 health sciences
biology
Chemistry
Genitourinary system
010401 analytical chemistry
Genitalia, Female
Cell Biology
Middle Aged
biology.organism_classification
Immunohistochemistry
0104 chemical sciences
Vaginal tissue
Nitric oxide synthase
Specific antibody
nervous system
Vagina
biology.protein
Female
Signal amplification
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 631
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....31932076d5b7f32c522ce90f7d03b797