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Gender comparisons in human lung cancer: analysis of p53 mutations, anti-p53 serum antibodies and C-erbB-2 expression
- Source :
- Carcinogenesis. 16(5)
- Publication Year :
- 1995
-
Abstract
- Little is known about the molecular mechanisms of lung carcinogenesis in women. We initiated an investigation of the role of gender in pulmonary carcinogenesis by analysis of p53 mutations, immunohistochemistry, serum antibodies and c-erbB-2 expression in a series of 63 male and 44 female lung cancer patients whose tumors were resected at the Mayo Clinic between 1991 and 1992. There were 102 smokers and 5 never smoked. Adenocarcinoma was the more frequent histological type in women (62%) than in men (41%). Sequence analysis of exons 5-8 in 42 females and 49 males identified 44 p53 mutations in 42 tumors (46%). Base substitution mutations showed a preponderance of G:C-->T:A transversions, which were more frequent in women than men (40 versus 25%) and in individuals exposed to asbestos. c-erbB-2 immunohistochemical staining was identified more frequently in females (nine cases) than males (two cases). Marked immunohistochemical staining for p53 positively correlated with the presence of missense mutations in exons 5-8 (81%, P < 0.001). Seven missense mutations (four in exon 5, two in exon 6, one in exon 8) were identified in five of nine patients who had serum antibodies recognizing p53; tumors from these patients were also strongly positive for p53 by immunohistochemistry. These and other results indicate gender differences in the genetic and biochemical alterations in lung cancer and generate hypothesis regarding gender differences in lung cancer susceptibility.
- Subjects :
- Male
Cancer Research
Pathology
medicine.medical_specialty
Lung Neoplasms
Receptor, ErbB-2
Immunoblotting
Gene Expression
Biology
Adenocarcinoma
medicine.disease_cause
Polymerase Chain Reaction
Immunoenzyme Techniques
Exon
medicine
Missense mutation
Humans
Point Mutation
Carcinoma, Small Cell
Lung cancer
Autoantibodies
Sex Characteristics
Respiratory disease
Smoking
Asbestos
General Medicine
DNA
DNA, Neoplasm
Exons
Genes, erbB-2
Middle Aged
medicine.disease
Genes, p53
Lung cancer susceptibility
Immunohistochemistry
Protein Biosynthesis
Carcinoma, Squamous Cell
Carcinoma, Large Cell
Female
Tumor Suppressor Protein p53
Carcinogenesis
Subjects
Details
- ISSN :
- 01433334
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Carcinogenesis
- Accession number :
- edsair.doi.dedup.....318c9c5a2b1936877874aa652494b13a