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Leptogenic effects of NAPE require activity of NAPE-hydrolyzing phospholipase D
- Source :
- Journal of Lipid Research, Vol 58, Iss 8, Pp 1624-1635 (2017)
- Publication Year :
- 2017
-
Abstract
- Food intake induces synthesis of N-acylphosphatidylethanolamines (NAPEs) in the intestinal tract. While NAPEs exert leptin-like (leptogenic) effects, including reduced weight gain and food intake, the mechanisms by which NAPEs induce these leptogenic effects remain unclear. One key question is whether intestinal NAPEs act directly on cognate receptors or first require conversion to N-acylethanolamides (NAEs) by NAPE-hydrolyzing phospholipase D (NAPE-PLD). Previous studies using Nape-pld−/− mice were equivocal because intraperitoneal injection of NAPEs led to nonspecific aversive effects. To avoid the aversive effects of injection, we delivered NAPEs and NAEs intestinally using gut bacteria synthesizing these compounds. Unlike in wild-type mice, increasing intestinal levels of NAPE using NAPE-synthesizing bacteria in Nape-pld−/− mice failed to reduce food intake and weight gain or alter gene expression. In contrast, increasing intestinal NAE levels in Nape-pld−/− mice using NAE-synthesizing bacteria induced all of these effects. These NAE-synthesizing bacteria also markedly increased NAE levels and decreased inflammatory gene expression in omental adipose tissue. These results demonstrate that intestinal NAPEs require conversion to NAEs by the action of NAPE-PLD to exert their various leptogenic effects, so that the reduced intestinal NAPE-PLD activity found in obese subjects may directly contribute to excess food intake and obesity.
- Subjects :
- 0301 basic medicine
Leptin
medicine.medical_specialty
obesity
Nape
medicine.medical_treatment
Intraperitoneal injection
Arabidopsis
Adipose tissue
QD415-436
Phospholipase
liver
Biochemistry
03 medical and health sciences
Mice
0302 clinical medicine
Endocrinology
Internal medicine
Gene expression
medicine
Phospholipase D
Animals
Receptor
Research Articles
adipose
Chemistry
Hydrolysis
Phosphatidylethanolamines
Cell Biology
030104 developmental biology
medicine.anatomical_structure
phosphatidylethanolamine
N-acylphosphatidylethanolamine
N-Acylphosphatidylethanolamine
030217 neurology & neurosurgery
diet effects/lipid metabolism
Subjects
Details
- ISSN :
- 15397262
- Volume :
- 58
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of lipid research
- Accession number :
- edsair.doi.dedup.....3188cfdf29d4f212ff123bedb4ffac47