Back to Search
Start Over
Toll-Like Receptors 2 and 3 Agonists Differentially Affect Oligodendrocyte Survival, Differentiation, and Myelin Membrane Formation
- Source :
- Journal of Neuroscience Research, 90(2), 388-398. Wiley-Blackwell
- Publication Year :
- 2012
-
Abstract
- Toll-like receptors (TLRs) play a key role in controlling innate immune responses to a wide variety of pathogen-associated molecules as well as endogenous signals. In addition, TLR expression within nonimmune cells has been recognized as as modulator of cell behavior. In this study we have addressed the question of whether functional TLRs are expressed on oligodendrocytes, the myelinating cells of the central nervous system. Primary cultures of rat oligodendrocytes at different maturation stages were found to express TLR2 and, to lesser extent, TLR3. Immunocytochemical analysis revealed that both TLRs were localized at the cell body and primary processes and were excluded from myelin-like membranes. Interestingly, innate immune receptor ligands were able to modulate oligodendrocyte survival, differentiation, and myelin-like membrane formation, indicating that TLRs on oligodendrocytes are functional. In highly purified oligodendrocytes cultures, the TLR2 agonist zymosan promoted survival, differentiation, and myelin-like membrane formation, whereas poly-I:C, a TLR3 ligand, was a potent inducer of apoptosis. Together, these data indicate that, in addition to other neural cell types, also oligodendrocytes express functional TLRs, which play a role in regulating various aspects of oligodendrocyte behavior. (C) 2011 Wiley Periodicals, Inc.
- Subjects :
- Cell Survival
Cell
LIPOPOLYSACCHARIDE
Biology
RIG-I
ACTIVATION
Cellular and Molecular Neuroscience
Myelin
INFLAMMATION
medicine
STRANDED-RNA
INJURY
Animals
Rats, Wistar
Receptor
Cells, Cultured
Myelin Sheath
Toll-like receptor
Innate immune system
Cell Membrane
CENTRAL-NERVOUS-SYSTEM
Zymosan
RECOGNITION
Cell Differentiation
NEGATIVE REGULATOR
Toll-Like Receptor 2
Oligodendrocyte
Rats
Toll-Like Receptor 3
Cell biology
Oligodendroglia
TLR2
myelin
medicine.anatomical_structure
Animals, Newborn
TLR3
INNATE IMMUNITY
toll-like receptor
oligodendrocyte
Subjects
Details
- Language :
- English
- ISSN :
- 03604012
- Volume :
- 90
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Neuroscience Research
- Accession number :
- edsair.doi.dedup.....3183fdae7cf14ba935b26aa8014ecd75