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Kinetic Profile of the Transcriptome Changes Induced in the Choroid Plexus by Peripheral Inflammation
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP
- Publication Year :
- 2009
- Publisher :
- SAGE Publications, 2009.
-
Abstract
- The choroid plexus, being part of the blood-brain barriers and responsible for the production of cerebrospinal fluid, is ideally positioned to transmit signals into and out of the brain. This study, using microarray analysis, shows that the mouse choroid plexus displays an acute-phase response after an inflammatory stimulus induced in the periphery by lipopolysaccharide (LPS). Remarkably, the response is specific to a restricted number of genes (out of a total of 24,000 genes analyzed, 252 are up-regulated and 173 are down-regulated) and transient, as it returns to basal conditions within 72 h. The up-regulated genes cluster into families implicated in immune-mediated cascades and in extracellular matrix remodeling, whereas those down-regulated participate in maintenance of the barrier function. Importantly, several acute-phase proteins, whose blood concentrations rise in response to inflammation, may contribute to the effects observed in vivo after LPS injection, as suggested by the differential response of primary choroid plexus epithelial cell cultures to LPS alone or to serum collected from animals exposed to LPS. By modulating the composition of the cerebrospinal fluid, which will ultimately influence the brain parenchyma, the choroid plexus response to inflammation may be of relevance in brain homeostasis in health and disease.<br />This work was supported by grant POCTI/SAU-NEU/ 56618/2004 from the Portuguese Foundation for Science and Technology (FCT) /FEDER; and from a grant from the DANA foundation; Marques F, Falcao AM, and Rodrigues AJ are recipients of fellowships from FCT/FEDER.
- Subjects :
- Lipopolysaccharides
Male
Down-Regulation
Gene Expression
Inflammation
Biology
Blood–brain barrier
Mice
03 medical and health sciences
0302 clinical medicine
Cerebrospinal fluid
Parenchyma
medicine
Animals
Rats, Wistar
Acute-Phase Reaction
Cells, Cultured
Barrier function
Oligonucleotide Array Sequence Analysis
030304 developmental biology
0303 health sciences
Science & Technology
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
lipopolysaccharide
Acute-phase protein
Epithelial Cells
Rats
Up-Regulation
3. Good health
Cell biology
Mice, Inbred C57BL
Kinetics
medicine.anatomical_structure
Neurology
Blood-Brain Barrier
Choroid Plexus
Choroid plexus
Neurology (clinical)
Choroid
medicine.symptom
Transcriptome
Cardiology and Cardiovascular Medicine
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15597016 and 0271678X
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Journal of Cerebral Blood Flow & Metabolism
- Accession number :
- edsair.doi.dedup.....3175f0c91d70c34b6e5ece33c014be56