Additional file 3 of Biochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase

Additional file 3: Supporting information. Table S1. Whole-genome sequencing using the Illumina MiniSeq® platform. Table S2. Plasmids constructed and used in this study. Figure S1. Expression and purification of Ecm14. Figure S2. Edman degradation spectra. Figure S3. Large-scale screens identifying phenotypes for ECM14 mutants are not reproducible. Figure S4. Map of pSLS1-ECM14. Figure S5. The effect of EMS exposure time on yeast cell viability. Figure S6. Putative synthetic lethal mutants have reduced fitness on minimal media.