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Paper biosensors for detecting elevated IL-6 levels in blood and respiratory samples from COVID-19 patients
- Source :
- Sensors and Actuators B: Chemical, Sensors and Actuators. B, Chemical
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Graphical abstract<br />Highlights • Detection of cytokine storm biomarkers with mobile biosensors. • Immunosensors are made solely of cellulose modified with antibody-decorated nanoparticles. • Turnaround time under 10 min. • Detection of biomarkers in respiratory samples allows monitoring local inflammation. • Compatible with decentralized health care schemes.<br />Decentralizing COVID-19 care reduces contagions and affords a better use of hospital resources. We introduce biosensors aimed at detecting severe cases of COVID-19 in decentralized healthcare settings. They consist of a paper immunosensor interfaced with a smartphone. The immunosensors have been designed to generate intense colorimetric signals when the sample contains ultralow concentrations of IL-6, which has been proposed as a prognosis biomarker of COVID-19. This is achieved by combining a paper-based signal amplification mechanism with polymer-filled reservoirs for dispensing antibody-decorated nanoparticles and a bespoken app for color quantification. With this design we achieved a low limit of detection (LOD) of 10−3 pg mL-1 and semi-quantitative measurements in a wide dynamic range between 10−3 and 102 pg mL-1 in PBS. The assay time is under 10 min. The low LOD allowed us to dilute blood samples and detect IL-6 with an LOD of 1.3 pg mL-1 and a dynamic range up to 102 pg mL-1. Following this protocol, we were able to stratify COVID-19 patients according to different blood levels of IL-6. We also report on the detection of IL-6 in respiratory samples (bronchial aspirate, BAS) from COVID-19 patients. The test could be easily adapted to detect other cytokines such as TNF-α and IL-8 by changing the antibodies decorating the nanoparticles accordingly. The ability of detecting cytokines in blood and respiratory samples paves the way for monitoring local inflammation in the lungs as well as systemic inflammation levels in the body.
- Subjects :
- Coronavirus disease 2019 (COVID-19)
Paper-based
02 engineering and technology
010402 general chemistry
Systemic inflammation
01 natural sciences
Article
Prognosis biomarker
Wide dynamic range
Materials Chemistry
Medicine
Electrical and Electronic Engineering
Interleukin 6
Respiratory samples
Instrumentation
ComputingMethodologies_COMPUTERGRAPHICS
Detection limit
IL-6
biology
SARS-CoV-2
business.industry
Metals and Alloys
COVID-19
021001 nanoscience & nanotechnology
Condensed Matter Physics
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
biology.protein
Smartphone
medicine.symptom
0210 nano-technology
business
Biosensor
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 09254005
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi.dedup.....315948e54ddf489cafc174a7bb0bbb67
- Full Text :
- https://doi.org/10.1016/j.snb.2020.129333