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Molecular cloning of estrogen receptor alpha (ERα; ESR1) of the Japanese giant salamander, Andrias japonicus
- Source :
- Molecular and Cellular Endocrinology. :84-94
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Estrogens are essential for normal reproductive activity in females and males and for ovarian differentiation during a critical developmental stage in many vertebrates. To understand the molecular mechanisms of estrogen action and to evaluate estrogen receptor ligand interactions in the Japanese giant salamander ( Andrias japonicus ), we isolated cDNA encoding the estrogen receptor (ER) from the liver. A full-length Japanese giant salamander ER cDNA (jgsER) was obtained using 5′ and 3′ rapid amplification cDNA ends (RACE). The deduced amino acid sequence of the jgsER showed high identity to the Xenopus ERα (ESR1) (77.7%). We have applied both the conventional ERE-luciferase reporter assay system and the GAL4-transactivation system to characterize this receptor. In two different transient transfection assay systems using mammalian cells, the jgsER protein displayed estrogen-dependent activation of transcription. The GAL4-transactivation system showed about 10-fold greater activity of the estrogen receptor by hormone when compared to the conventional ERE-luciferase reporter assay system. Tissue distribution of ERα mRNA was examined and kidney, ovary and liver exhibited expression. This is the first isolation of an estrogen receptor from a salamander and also is the first functional cDNA obtained from the Japanese giant salamander, an endangered species considered a special natural monument of Japan.
- Subjects :
- Transcriptional Activation
medicine.drug_class
Molecular Sequence Data
Urodela
Estrogen receptor
Japanese giant salamander
CHO Cells
Biology
Transfection
Biochemistry
Estrogen-related receptor alpha
Endocrinology
Cricetinae
Complementary DNA
medicine
Animals
Tissue Distribution
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Phylogeny
Estrogen receptor beta
Base Sequence
Sequence Homology, Amino Acid
Estrogen Receptor alpha
biology.organism_classification
Molecular biology
Protein Structure, Tertiary
Estrogen
Female
Estrogen-related receptor gamma
Estrogen receptor alpha
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 03037207
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Endocrinology
- Accession number :
- edsair.doi.dedup.....3156ef056c15f2bbf105c4ba3cab6cf6