Back to Search Start Over

Complement activation by carbon nanotubes and its influence on the phagocytosis and cytokine response by macrophages

Authors :
Uday Kishore
Robert B. Sim
Anthony G. Tsolaki
Bennie ten Haken
Anne Jäkel
Silke Hampel
M.T. Sobik
Annapurna Nayak
Kirsten M. Pondman
Emmanuel Flahaut
University of Twente [Netherlands]
Brunel University London [Uxbridge]
University of Oxford [Oxford]
Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT)
Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3)
Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP)
Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)
Leibniz Institute for Solid State and Materials Research (IFW Dresden)
Leibniz Association
Kingston University (UNITED KINGDOM)
Centre National de la Recherche Scientifique - CNRS (FRANCE)
Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Université Toulouse III - Paul Sabatier - UT3 (FRANCE)
Brunel University (UNITED KINGDOM)
Leibniz-Institut für Festkörper- und Werkstoffforschung (GERMANY)
University of Oxford (UNITED KINGDOM)
University of Twente (NETHERLANDS)
Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux - CIRIMAT (Toulouse, France)
Magnetic Detection and Imaging
Faculty of Science and Technology
Institut National Polytechnique de Toulouse - INPT (FRANCE)
Source :
Nanomedicine: Nanotechnology, Biology and Medicine, Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2014, vol. 10 (n° 6), pp. 1287-1299. ⟨10.1016/j.nano.2014.02.010⟩, Nanomedicine : nanotechnology, biology and medicine, 10(6), 1287-1299. Elsevier
Publication Year :
2014
Publisher :
Elsevier BV, 2014.

Abstract

Carbon nanotubes (CNTs) have promised a range of applications in biomedicine. Although influenced by the dispersants used, CNTs are recognized by the innate immune system, predominantly by the classical pathway of the complement system. Here, we confirm that complement activation by the CNT used here continues up to C3 and C5, indicating that the entire complement system is activated including the formation of membrane-attack complexes. Using recombinant forms of the globular regions of human C1q (gC1q) as inhibitors of CNT-mediated classical pathway activation, we show that C1q, the first recognition subcomponent of the classical pathway, binds CNTs via the gC1q domain. Complement opsonisation of CNTs significantly enhances their uptake by U937 cells, with concomitant downregulation of pro-inflammatory cytokines and up-regulation of anti-inflammatory cytokines in both U937 cells and human monocytes. We propose that CNT-mediated complement activation may cause recruitment of cellular infiltration, followed by phagocytosis without inducing a pro-inflammatory immune response. Carbon nanotubes (CNTs) are recognized by the innate immune system, especially by the classical pathway of the complement system. Complement activation is usually associated with a pro-inflammatory response. Here, we show that complement deposition on CMC- and RNA-CNTs can enhance their uptake by phagocytes, which in turn, leads to dampening of pro-inflammatory cytokine production. It appears that CNT-mediated complement activation may cause recruitment of cellular infiltration, followed by enhanced phagocytosis and suppression of cytokine storm.

Details

ISSN :
15499634
Volume :
10
Database :
OpenAIRE
Journal :
Nanomedicine: Nanotechnology, Biology and Medicine
Accession number :
edsair.doi.dedup.....314fec027ebca9a23ace7e9ef8b54b7f
Full Text :
https://doi.org/10.1016/j.nano.2014.02.010