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Premature termination ofGAT1transcription explains paradoxical negative correlation between nitrogen-responsive mRNA, but constitutive low-level protein production
- Source :
- RNA Biology
- Publication Year :
- 2015
- Publisher :
- Informa UK Limited, 2015.
-
Abstract
- The first step in executing the genetic program of a cell is production of mRNA. In yeast, almost every gene is transcribed as multiple distinct isoforms, differing at their 5′ and/or 3′ termini. However, the implications and functional significance of the transcriptome-wide diversity of mRNA termini remains largely unexplored. In this paper, we show that the GAT1 gene, encoding a transcriptional activator of nitrogen-responsive catabolic genes, produces a variety of mRNAs differing in their 5′ and 3′ termini. Alternative transcription initiation leads to the constitutive, low level production of 2 full length proteins differing in their N-termini, whereas premature transcriptional termination generates a short, highly nitrogen catabolite repression- (NCR-) sensitive transcript that, as far as we can determine, is not translated under the growth conditions we used, but rather likely protects the cell from excess Gat1.
- Subjects :
- termination
Gene isoform
Saccharomyces cerevisiae Proteins
Nitrogen
Blotting, Western
Molecular Sequence Data
Saccharomyces cerevisiae
yeast
Biology
GATA Transcription Factors
Transcription (biology)
Gene Expression Regulation, Fungal
Protein biosynthesis
Amino Acid Sequence
RNA, Messenger
3' Untranslated Regions
Molecular Biology
Gene
Phylogeny
Genetics
Messenger RNA
Base Sequence
Models, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Three prime untranslated region
Alternative splicing
Cell Biology
Blotting, Northern
initiation
Alternative Splicing
GAT1
Protein Biosynthesis
Transcription Termination, Genetic
GATA transcription factor
Erratum
transcription
5' Untranslated Regions
Research Paper
Subjects
Details
- ISSN :
- 15558584 and 15476286
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- RNA Biology
- Accession number :
- edsair.doi.dedup.....313cec8ae430c84b2cb5cea087c85dc2